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Proteomics Reveal Enhanced Oxidative Stress Responses and Metabolic Adaptation in Acidithiobacillus ferrooxidans Biofilm Cells on Pyrite
Linnaeus University, Faculty of Health and Life Sciences, Department of Biology and Environmental Science. Univ Duisburg Essen, Germany. (Ctr Ecol & Evolut Microbial Model Syst EEMiS)
TU Bergakademie Freiberg, Germany.
Ruhr Univ Bochum, Germany;Univ Plymouth, UK.
Univ Duisburg Essen, Germany;TU Bergakad Freiberg, Germany;Donghua Univ, Peoples Republic of China.
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2019 (English)In: Frontiers in Microbiology, ISSN 1664-302X, E-ISSN 1664-302X, Vol. 10, p. 1-14, article id 592Article in journal (Refereed) Published
Abstract [en]

Reactive oxygen species (ROS) cause oxidative stress and growth inhibition by inactivation of essential enzymes, DNA and lipid damage in microbial cells. Acid mine drainage (AMD) ecosystems are characterized by low pH values, enhanced levels of metal ions and low species abundance. Furthermore, metal sulfides, such as pyrite and chalcopyrite, generate extracellular ROS upon exposure to acidic water. Consequently, oxidative stress management is especially important in acidophilic leaching microorganisms present in industrial biomining operations, especially when forming biofilms on metal sulfides. Several adaptive mechanisms have been described, but the molecular repertoire of responses upon exposure to pyrite and the presence of ROS are not thoroughly understood in acidophiles. In this study the impact of the addition of H2O2 on iron oxidation activity in Acidithiobacillus ferrooxidans DSM 14882(T) was investigated. Iron(II)- or sulfur-grown cells showed a higher sensitivity toward H2O2 than pyrite-grown ones. In order to elucidate which molecular responses may be involved, we used shot-gun proteomics and compared proteomes of cells grown with iron(II)-ions against biofilm cells, grown for 5 days in presence of pyrite as sole energy source. In total 1157 proteins were identified. 213 and 207 ones were found to have increased levels in iron(II) ion-grown or pyrite-biofilm cells, respectively. Proteins associated with inorganic sulfur compound (ISC) oxidation were among the latter. In total, 80 proteins involved in ROS degradation, thiol redox regulation, macromolecule repair mechanisms, biosynthesis of antioxidants, as well as metal and oxygen homeostasis were found. 42 of these proteins had no significant changes in abundance, while 30 proteins had increased levels in pyrite-biofilm cells. New insights in ROS mitigation strategies, such as importance of globins for oxygen homeostasis and prevention of unspecific reactions of free oxygen that generate ROS are presented for A. ferrooxidans biofilm cells. Furthermore, proteomic analyses provide insights in adaptations of carbon fixation and oxidative phosphorylation pathways under these two growth conditions.

Place, publisher, year, edition, pages
Frontiers Media S.A., 2019. Vol. 10, p. 1-14, article id 592
Keywords [en]
Acidithiobacillus ferrooxidans, proteomics, bioleaching, oxidative stress, reactive oxygen species, biofilm formation, pyrite
National Category
Microbiology Ecology
Research subject
Ecology, Microbiology
Identifiers
URN: urn:nbn:se:lnu:diva-81845DOI: 10.3389/fmicb.2019.00592ISI: 000462717400001Scopus ID: 2-s2.0-85066618844OAI: oai:DiVA.org:lnu-81845DiVA, id: diva2:1304333
Available from: 2019-04-12 Created: 2019-04-12 Last updated: 2019-08-29Bibliographically approved

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