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Assembly of functionalized silk together with cells to obtain proliferative 3D cultures integrated in a network of ECM-like microfibers
Linnaeus University, Faculty of Health and Life Sciences, Department of Chemistry and Biomedical Sciences. KTH Royal Instute of Technology, Sweden;Uppsala University, Sweden. (Linnaeus Ctr Biomat Chem, BMC)
KTH Royal Instute of Technology, Sweden.
KTH Royal Instute of Technology, Sweden.
KTH Royal Instute of Technology, Sweden.
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2019 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 9, p. 1-13, article id 6291Article in journal (Refereed) Published
Abstract [en]

Tissues are built of cells integrated in an extracellular matrix (ECM) which provides a three-dimensional (3D) microfiber network with specific sites for cell anchorage. By genetic engineering, motifs from the ECM can be functionally fused to recombinant silk proteins. Such a silk protein, FN-silk, which harbours a motif from fibronectin, has the ability to self-assemble into networks of microfibers under physiological-like conditions. Herein we describe a method by which mammalian cells are added to the silk solution before assembly, and thereby get uniformly integrated between the formed microfibers. In the resulting 3D scaffold, the cells are highly proliferative and spread out more efficiently than when encapsulated in a hydrogel. Elongated cells containing filamentous actin and defined focal adhesion points confirm proper cell attachment to the FN-silk. The cells remain viable in culture for at least 90 days. The method is also scalable to macro-sized 3D cultures. Silk microfibers formed in a bundle with integrated cells are both strong and extendable, with mechanical properties similar to that of artery walls. The described method enables differentiation of stem cells in 3D as well as facile co-culture of several different cell types. We show that inclusion of endothelial cells leads to the formation of vessel-like structures throughout the tissue constructs. Hence, silk-assembly in presence of cells constitutes a viable option for 3D culture of cells integrated in a ECM-like network, with potential as base for engineering of functional tissue.

Place, publisher, year, edition, pages
Nature Publishing Group, 2019. Vol. 9, p. 1-13, article id 6291
National Category
Cell Biology
Research subject
Natural Science, Cell and Organism Biology
Identifiers
URN: urn:nbn:se:lnu:diva-82537DOI: 10.1038/s41598-019-42541-yISI: 000465001600045PubMedID: 31000733Scopus ID: 2-s2.0-85064539422OAI: oai:DiVA.org:lnu-82537DiVA, id: diva2:1315651
Available from: 2019-05-14 Created: 2019-05-14 Last updated: 2019-08-29Bibliographically approved

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Johansson, Ulrika

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