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Undersökning av genuttrycket av proteorhodopsin i Dokdonia donghaensis MED134 med qPCR-analyser
Linnaeus University, Faculty of Health and Life Sciences, Department of Chemistry and Biomedical Sciences.
2019 (Swedish)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [sv]

Dokdonia donghaensis MED134 är en marin bakterie som använder kol och kväve för sin energikälla. Dessa bakterier har också förmågan att skapa energi från ljus med hjälp av en membranbunden protonpump som kallas för proteorhodopsin. I detta arbete användes qPCR-analyser (quantitative polymerase chain reaction) i två olika experiment, koloni- och evolutionsexperiment. Syftet med koloniexperimenten var att jämföra proteorhodopsin uttrycket i D. donghaensis MED134 i kolonier från primär- och tertiärstryk på en platta som har fått växa i ljus respektive mörker under tre respektive sju dagar. Syftet med evolutionsexperimenten var att undersöka bakterietillväxten och jämföra uttrycket av proteorhodopsin i generation 130 av D. donghaensis MED134 med ursprungsprovet (generation 0) som har fått växa i ljus och mörker i ett flytande medium. Genexpressionsanalysen visade att kolonierna som växte vid primärstryket (ljus) hade tendens till högre proteorhodopsin uttryck jämfört med tertiärstryket (ljus) vid dag 3. Proteorhodopsin uttrycket i ljus ökade i tertiärstryket vid dag 7. I evolutionsexperimenten hade generation 0 och 130 (ljus) högre tillväxt än de som växte i mörker. Generation 130 (ljus) hade tendens till högre proteorhodopsin uttryck jämfört med alla andra, och generation 130 i mörker hade mindre proteorhodopsin uttryck än generation 0 i mörker. Statistisk test visade ingen signifikant skillnad mellan de olika grupperna i båda experimenten. Resultat i denna studie indikerar att uttrycket av proteorhodopsin varierar beroende på tid och var i plattan bakterierna växer, då konkurrensen spelar stor roll. Evolutionen kan också ha bidragit till bättre överlevnad för generation 130 (ljus) och sämre för generation 130 (mörker).

Abstract [en]

Dokdonia donghaensis MED134 is a marine bacterium that uses carbon and nitrogen for its energy source. These bacteria also have the ability to generate energy from light by using a membrane-bound proton pump called proteorhodopsin. In this work, qPCR analysis (quantitative polymerase chain reaction) was used in two different experiments, colony and evolution experiment. The aim of the colony experiment was to compare the proteorhodopsin expression in D. donghaensis MED134 in colonies from primary- and tertiary streak on a plate that has been allowed to grow in light and dark for three and seven days. The purpose of the evolution experiment was to investigate the bacterial growth and compare the expression of proteorhodopsin in generation 130 in D. donghaensis MED134 with the wild type sample (generation 0) which has grown in light and dark in a liquid medium. The results of the gene expression analysis showed that the colonies that grew at the primary streak (light), had tendency to higher proteorhodopsin expression compared to the tertiary streak (light) at day 3. The proteorhodopsin expression in light increased in the tertiary streak at day 7. In the evolution experiment, generation 0 and 130 (light) had higher growth than those that grew in the dark. Generation 130 (light) had tendency to higher proteorhodopsin expression compared to all others, and generation 130 in the dark had less proteorhodopsin expression than generation 0 in the dark. Statistical tests showed no significant difference between the groups in both experiments. The result in this study indicate that the expression of proteorhodopsin varies depending on time and where on the plate the bacteria have grown since competition plays a major role. Evolution may also have contributed to better survival for generation 130 (light) and worse for generation 130 (dark).

Place, publisher, year, edition, pages
2019. , p. 37
Keywords [sv]
Dokdonia donghaensis MED134, Proteorhodopsin, qPCR
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:lnu:diva-87948OAI: oai:DiVA.org:lnu-87948DiVA, id: diva2:1343256
Subject / course
Biomedical Laboratory Science
Educational program
Biomedical Laboratory Science Programme, 180 credits
Supervisors
Examiners
Available from: 2019-08-16 Created: 2019-08-15 Last updated: 2019-08-16Bibliographically approved

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CiteExportLink to record
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Citation style
  • apa
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