Timber-to-timber connections with different types of screws and nails were experimentally investigated with the aim to quantify the contribution of their axial resistance to their slip and their lateral load bearing capacity, which is the so-called rope effect in dowel-type timber connections. Five different types of screws, including partly threaded and double-threaded screws, as well as three types of nails, including smooth round nails, twisted square-sectioned and grooved nails, were used in the experimental investigations in order to cover a broad range of axial resistance of fasteners. Their behavior was tested in single shear and double shear timber-to-timber connections. In addition to connection testing, system and material properties were experimentally determined. This gave input to design equations and allowed for a comparison of the mechanical model in the European design standard for timber structures, Eurocode 5, with experiments. Experiments indicate that the initial slip modulus in the quasi-elastic domain is not influenced by withdrawal capacity of the fastener, while it shows pronounced influence on load-carrying capacity and the nonlinear shape of the slip curve of the connection at larger deformations. Rope effect strongly depends on fastener properties. High axial resistance of fasteners leads to highly non-linear slip curves and design equations tended to underestimate strength including the rope effect up to a displacement limit of 15 mm. Experimentally observed failure modes were well in line with the theoretical failure modes predicted by the design model. The provided experimental results are motivation for improvement of design models and development of analytical and numerical models that account for nonlinear effects in the complex load transfer mechanism.