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Regeneration of Assembled, Molecular-Motor-Based Bionanodevices
Linnaeus University, Faculty of Health and Life Sciences, Department of Chemistry and Biomedical Sciences. Lund University, Sweden.ORCID iD: 0000-0002-2797-2294
Tech Univ Dresden, Germany;Max Planck Inst Mol Cell Biol & Genet, Germany.
Lund University, Sweden.ORCID iD: 0000-0001-5617-6337
Tech Univ Dresden, Germany;Max Planck Inst Mol Cell Biol & Genet, Germany.
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2019 (English)In: Nano letters (Print), ISSN 1530-6984, E-ISSN 1530-6992, Vol. 19, no 10, p. 7155-7163Article in journal (Refereed) Published
Abstract [en]

The guided gliding of cytoskeletal filaments, driven by biomolecular motors on nano/microstructured chips, enables novel applications in biosensing and biocomputation. However, expensive and time-consuming chip production hampers the developments. It is therefore important to establish protocols to regenerate the chips, preferably without the need to dismantle the assembled microfluidic devices which contain the structured chips. We here describe a novel method toward this end. Specifically, we use the small, nonselective proteolytic enzyme, proteinase K to cleave all surface-adsorbed proteins, including myosin and kinesin motors. Subsequently, we apply a detergent (5% SDS or 0.05% Triton X100) to remove the protein remnants. After this procedure, fresh motor proteins and filaments can be added for new experiments. Both, silanized glass surfaces for actin-myosin motility and pure glass surfaces for microtubule-kinesin motility were repeatedly regenerated using this approach. Moreover, we demonstrate the applicability of the method for the regeneration of nano/microstructured silicon-based chips with selectively functionalized areas for supporting or suppressing gliding motility for both motor systems. The results substantiate the versatility and a promising broad use of the method for regenerating a wide range of protein-based nano/microdevices.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2019. Vol. 19, no 10, p. 7155-7163
Keywords [en]
Nano/microdevice, regeneration, protein desorption, molecular motor, proteinase K, detergent
National Category
Biochemistry and Molecular Biology
Research subject
Chemistry, Biochemistry
Identifiers
URN: urn:nbn:se:lnu:diva-89868DOI: 10.1021/acs.nanolett.9b02738ISI: 000490353500058PubMedID: 31512480OAI: oai:DiVA.org:lnu-89868DiVA, id: diva2:1366935
Available from: 2019-10-31 Created: 2019-10-31 Last updated: 2019-10-31Bibliographically approved

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Rahman, Mohammad A.Salhotra, AseemMånsson, Alf

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