We present results from a unique nationwide survey conducted in Sweden on sexual orientation and job satisfaction. Our results show that gay men are more likely to be very satisfied with their job than heterosexual men, both in general and with different aspects of the job. In contrast, lesbians appear less satisfied with their job in general and with promotion prospects than heterosexual women. Among women, we find no differences in the probability of being very satisfied with the job by sexual orientation. However, the issue of sexual orientation and job satisfaction is complex since gay men, despite that they more often are very satisfied with the job, like lesbians find their job more mentally straining than heterosexuals. We conclude that gay men and lesbians are facing other stressers at work than heterosexuals do. We also conclude that the worker’s own expectations about the job and possibilities to fulfill career plans may be possible driving forces to our results. Previous research has documented discrimination against gay males and lesbians in hiring. Our results underline that measures to prevent discrimination in hiring is not enough to create an equal working life for homosexuals and heterosexuals. Measures within the workplace are also needed.