Optimization of Islet Microencapsulation with Thin Polymer Membranes for Long-Term StabilityShow others and affiliations
2019 (English)In: Micromachines, E-ISSN 2072-666X, Vol. 10, no 11, p. 1-10, article id 755Article in journal (Refereed) Published
Abstract [en]
Microencapsulation of islets can protect against immune reactions from the host immune system after transplantation. However, sufficient numbers of islets cannot be transplanted due to the increase of the size and total volume. Therefore, thin and stable polymer membranes are required for the microencapsulation. Here, we undertook the cell microencapsulation using poly(ethylene glycol)-conjugated phospholipid (PEG-lipid) and layer-by-layer membrane of multiple-arm PEG. In order to examine the membrane stability, we used different molecular weights of 4-arm PEG (10k, 20k and 40k)-Mal to examine the influence on the polymer membrane stability. We found that the polymer membrane made of 4-arm PEG(40k)-Mal showed the highest stability on the cell surface. Also, the polymer membrane did not disturb the insulin secretion from beta cells.
Place, publisher, year, edition, pages
MDPI, 2019. Vol. 10, no 11, p. 1-10, article id 755
Keywords [en]
microencapsulation, bioartificial pancreas, islet transplantation, polyethylene glycol-lipid (PEG-lipid), cell surface modification
National Category
Immunology Biochemistry and Molecular Biology
Research subject
Biomedical Sciences, Immunology
Identifiers
URN: urn:nbn:se:lnu:diva-90843DOI: 10.3390/mi10110755ISI: 000502255300041PubMedID: 31698737Scopus ID: 2-s2.0-85075581733OAI: oai:DiVA.org:lnu-90843DiVA, id: diva2:1384745
2020-01-102020-01-102024-01-17Bibliographically approved