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The Difference of Serum Protein Transport between Echinosides and Verbascoside
Zhejiang Agr & Forestry Univ, China.
Zhejiang Agr & Forestry Univ, China.
Linnaeus University, Faculty of Health and Life Sciences, Department of Chemistry and Biomedical Sciences.ORCID iD: 0000-0001-8899-5046
Zhejiang Agr & Forestry Univ, China.
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2020 (English)In: Journal of the Chemical Society of Pakistan, ISSN 0253-5106, Vol. 42, no 3, p. 369-382Article in journal (Refereed) Published
Abstract [en]

Verbascoside (VER) is the enzymatic hydrolysis product of echinacoside (ECH). The molecular structures of ECH and VER have different glucosyl groups so they bind to serum albumin in different ways, resulting in different pharmacological actions. In this report, we have examined the binding characteristics between human serum albumin (HSA) and ECH/VER by molecular modeling and spectroscopic approaches. Molecular modeling revealed that VER bound to HSA mainly through hydrogen bonds, van der Waals forces and hydrophobic forces. The spectroscopic results showed that the interactions between HSA and VER/ECH involved a static binding process, and the bonding strength of the VER-HSA complex was stronger than that of the ECH-HSA complex. The value of the binding distances (r) was low, which indicated the occurrence of energy transfer. The reaction conformational pattern of HSA-VER and HSA-ECH gave a "two-state model" based on fluorescent phase diagram analysis. According to the thermodynamic model, the main forces between interaction of VER and HSA were hydrogen bonds and van der Waals forces, whereas the interaction between ECH and HSA was hydrophobic force. The fluorescence polarization analysis demonstrated that the interaction between HSA and VER or ECH generated a non-covalent complex. Compared with ECH, VER was more likely to bind with HSA because of its smaller molecular size and low polarity. The results of the spectral analysis concurred with the molecular modeling data, which provides a helpful reference for the study of the molecular reaction mechanism of VER/ECH binding to HSA.

Place, publisher, year, edition, pages
The chemical Society of Pakistan , 2020. Vol. 42, no 3, p. 369-382
Keywords [en]
Verbascoside, Echinacosid, Serum, Albumin, Spectrum, Experiment, Molecular modeling
National Category
Biochemistry and Molecular Biology
Research subject
Chemistry, Biochemistry
Identifiers
URN: urn:nbn:se:lnu:diva-97728ISI: 000549342800004Scopus ID: 2-s2.0-85097898817OAI: oai:DiVA.org:lnu-97728DiVA, id: diva2:1461721
Available from: 2020-08-27 Created: 2020-08-27 Last updated: 2021-05-11Bibliographically approved

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Brodelius, Peter E.

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