Combined Methylome, Transcriptome and Proteome Analyses Document Rapid Acclimatization of a Bacterium to Environmental ChangesShow others and affiliations
2020 (English)In: Frontiers in Microbiology, E-ISSN 1664-302X, Vol. 11, p. 1-21, article id 544785
Article in journal (Refereed) Published
Abstract [en]
Polynucleobacter asymbioticusstrain QLW-P1DMWA-1(T)represents a group of highly successful heterotrophic ultramicrobacteria that is frequently very abundant (up to 70% of total bacterioplankton) in freshwater habitats across all seven continents. This strain was originally isolated from a shallow Alpine pond characterized by rapid changes in water temperature and elevated UV radiation due to its location at an altitude of 1300 m. To elucidate the strain's adjustment to fluctuating environmental conditions, we recorded changes occurring in its transcriptomic and proteomic profiles under contrasting experimental conditions by simulating thermal conditions in winter and summer as well as high UV irradiation. To analyze the potential connection between gene expression and regulation via methyl group modification of the genome, we also analyzed its methylome. The methylation pattern differed between the three treatments, pointing to its potential role in differential gene expression. An adaptive process due to evolutionary pressure in the genus was deduced by calculating the ratios of non-synonymous to synonymous substitution rates for 20Polynucleobacterspp. genomes obtained from geographically diverse isolates. The results indicate purifying selection.
Place, publisher, year, edition, pages
Frontiers Media S.A., 2020. Vol. 11, p. 1-21, article id 544785
Keywords [en]
DNA modification, gene expression, freshwater heterotrophic bacteria, UV radiation, purifying selection
National Category
Microbiology
Research subject
Ecology, Microbiology
Identifiers
URN: urn:nbn:se:lnu:diva-98627DOI: 10.3389/fmicb.2020.544785ISI: 000576081900001PubMedID: 33042055Scopus ID: 2-s2.0-85091783770OAI: oai:DiVA.org:lnu-98627DiVA, id: diva2:1478933
2020-10-232020-10-232024-01-17Bibliographically approved