lnu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Combined Methylome, Transcriptome and Proteome Analyses Document Rapid Acclimatization of a Bacterium to Environmental Changes
Leibniz Inst Freshwater Ecol & Inland Fisheries, Germany;Univ Vienna, Austria.
Free Univ Berlin, Germany;SRM Univ AP, India.
Univ Vienna, Austria.
Japan Agcy Marine Earth Sci & Technol, Japan.
Show others and affiliations
2020 (English)In: Frontiers in Microbiology, E-ISSN 1664-302X, Vol. 11, p. 1-21, article id 544785Article in journal (Refereed) Published
Abstract [en]

Polynucleobacter asymbioticusstrain QLW-P1DMWA-1(T)represents a group of highly successful heterotrophic ultramicrobacteria that is frequently very abundant (up to 70% of total bacterioplankton) in freshwater habitats across all seven continents. This strain was originally isolated from a shallow Alpine pond characterized by rapid changes in water temperature and elevated UV radiation due to its location at an altitude of 1300 m. To elucidate the strain's adjustment to fluctuating environmental conditions, we recorded changes occurring in its transcriptomic and proteomic profiles under contrasting experimental conditions by simulating thermal conditions in winter and summer as well as high UV irradiation. To analyze the potential connection between gene expression and regulation via methyl group modification of the genome, we also analyzed its methylome. The methylation pattern differed between the three treatments, pointing to its potential role in differential gene expression. An adaptive process due to evolutionary pressure in the genus was deduced by calculating the ratios of non-synonymous to synonymous substitution rates for 20Polynucleobacterspp. genomes obtained from geographically diverse isolates. The results indicate purifying selection.

Place, publisher, year, edition, pages
Frontiers Media S.A., 2020. Vol. 11, p. 1-21, article id 544785
Keywords [en]
DNA modification, gene expression, freshwater heterotrophic bacteria, UV radiation, purifying selection
National Category
Microbiology
Research subject
Ecology, Microbiology
Identifiers
URN: urn:nbn:se:lnu:diva-98627DOI: 10.3389/fmicb.2020.544785ISI: 000576081900001PubMedID: 33042055Scopus ID: 2-s2.0-85091783770OAI: oai:DiVA.org:lnu-98627DiVA, id: diva2:1478933
Available from: 2020-10-23 Created: 2020-10-23 Last updated: 2024-01-17Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedScopus

Authority records

Hötzinger, Matthias

Search in DiVA

By author/editor
Hötzinger, Matthias
By organisation
Department of Biology and Environmental Science
In the same journal
Frontiers in Microbiology
Microbiology

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 24 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf