Tourism plays an important role in biotic exchange. It has the potential to become one of the most salient vectors of bio-contaminants on Earth. Through the destinations visited, the activities undertaken, the types of transportation used and other variables, tourism can be a significant pathway for biological invasions. In particular, this chapter looks at the role of aviation, rural and ecotourism, remoteness of the destination in relation to urban areas and other established destinations, increased contact between animals and humans, environmental change (e.g. building roads), and human contact with disease vectors (e.g. flies and mosquitos). Common biohazard vectors and pathways include luggage, food, shoes and clothing, modes of transportation and the like. The chapter also discusses ways of managing biological invasions and other biosecurity concerns.