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Structure and function of virion RNA polymerase of a crAss-like phage
Skolkovo Inst Sci & Technol, Russia.
Skolkovo Inst Sci & Technol, Russia;Russian Acad Sci, Russia.
Skolkovo Inst Sci & Technol, Russia.
Skolkovo Inst Sci & Technol, Russia;Russian Acad Sci, Russia.
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2021 (English)In: Nature, ISSN 0028-0836, E-ISSN 1476-4687, Vol. 589, no 7841, p. 306-309Article in journal (Refereed) Published
Sustainable development
Not refering to any SDG
Abstract [en]

The RNA polymerase from the crAss-like bacteriophage phi14:2, which is translocated into the host cell with phage DNA and transcribes early phage genes, is structurally most similar to eukaryotic RNA interference polymerases, suggesting that the latter have a phage origin. CrAss-like phages are a recently described expansive group of viruses that includes the most abundant virus in the human gut(1-3). The genomes of all crAss-like phages encode a large virion-packaged protein(2,4) that contains a DFDxD sequence motif, which forms the catalytic site in cellular multisubunit RNA polymerases (RNAPs)(5). Here, using Cellulophaga baltica crAss-like phage phi14:2 as a model system, we show that this protein is a DNA-dependent RNAP that is translocated into the host cell along with the phage DNA and transcribes early phage genes. We determined the crystal structure of this 2,180-residue enzyme in a self-inhibited state, which probably occurs before virion packaging. This conformation is attained with the help of a cleft-blocking domain that interacts with the active site and occupies the cavity in which the RNA-DNA hybrid binds. Structurally, phi14:2 RNAP is most similar to eukaryotic RNAPs that are involved in RNA interference(6,7), although most of the phi14:2 RNAP structure (nearly 1,600 residues) maps to a new region of the protein fold space. Considering this structural similarity, we propose that eukaryal RNA interference polymerases have their origins in phage, which parallels the emergence of the mitochondrial transcription apparatus(8).

Place, publisher, year, edition, pages
Nature Publishing Group, 2021. Vol. 589, no 7841, p. 306-309
National Category
Biochemistry and Molecular Biology
Research subject
Chemistry, Biochemistry
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URN: urn:nbn:se:lnu:diva-99928DOI: 10.1038/s41586-020-2921-5ISI: 000591047800001PubMedID: 33208949Scopus ID: 2-s2.0-85096237343Local ID: 2020OAI: oai:DiVA.org:lnu-99928DiVA, id: diva2:1517542
Available from: 2021-01-14 Created: 2021-01-14 Last updated: 2023-02-02Bibliographically approved

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Nilsson, EmelieHolmfeldt, Karin

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Yakunina, Maria V.Nilsson, EmelieHolmfeldt, Karin
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