Diagnostic challenges for the detection of emerging pathogens: A case study involving the incursion of Pseudomonas syringae pv. actinidiae in New ZealandShow others and affiliations
2014 (English)In: Detection and Diagnostics of Plant Pathogens: (Contributions to the 9th International Congress) / [ed] Gullino M., Bonants P., Springer, 2014, p. 71-86Chapter in book (Other academic)
Abstract [en]
In November 2010, Pseudomonas syringae pv. actinidiae (Psa) was detected for the first time in New Zealand. This finding triggered one of the largest surveillance and diagnostic programmes seen in New Zealandâs horticultural industry. During this response, over 912 kiwifruit orchards and 14,500 samples were screened and tested for the presence of Psa. The initial objectives of the response were to confirm the causal agent, determine disease prevalence and identify possible mechanisms of spread with the aim of identifying management options to contain the outbreak. Molecular diagnoses and characterisation of the Psa strains isolated during the response was conducted using a range of techniques that included qPCR, rep-PCR fingerprinting, multilocus sequence analysis, and next generation sequencing. The usefulness and challenges of using the molecular techniques available at the time for Psa detection and characterisation during the response are discussed. © Springer Science+Business Media Dordrecht 2014.
Place, publisher, year, edition, pages
Springer, 2014. p. 71-86
Series
Plant Pathology in the 21st Century ; 5
Keywords [en]
Bacteria; Molecular biology; Plasma diagnostics; Polymerase chain reaction; Space surveillance, Actinidia spp; Horticultural industry; Molecular techniques; Multilocus sequence analysis; New zealand; Next-generation sequencing; Pseudomonas syringae; Response, Diagnosis
National Category
Microbiology
Research subject
Ecology, Microbiology
Identifiers
URN: urn:nbn:se:lnu:diva-101370DOI: 10.1007/978-94-017-9020-8_5Scopus ID: 2-s2.0-84974836976ISBN: 9789401790208 (print)ISBN: 9789401790192 (print)OAI: oai:DiVA.org:lnu-101370DiVA, id: diva2:1531344
2021-02-252021-02-252021-02-25Bibliographically approved