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Phenotypic detection of clonotypic B cells in multiple myeloma by specific immunoglobulin ligands reveals their rarity in multiple myeloma
University Medical Center Hamburg-Eppendorf, Germany.
University Medical Center Hamburg-Eppendorf, Germany.
University Medical Center Freiburg, Germany.
University Medical Center Hamburg-Eppendorf, Germany.ORCID iD: 0000-0002-2132-2709
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2012 (English)In: PLOS ONE, E-ISSN 1932-6203, Vol. 7, no 2, article id e31998Article in journal (Refereed) Published
Abstract [en]

In multiple myeloma, circulating “clonotypic” B cells, that express the immunoglobulin rearrangement of the malignant plasma cell clone, can be indirectly detected by PCR. Their role as potential “feeder” cells for the malignant plasma cell pool remains controversial. Here we established for the first time an approach that allows direct tracking of such clonotypic cells by labeling with patient-specific immunoglobulin ligands in 15 patients with myeloma. Fifty percent of patients showed evidence of clonotypic B cells in blood or bone marrow by PCR. Epitope-mimicking peptides from random libraries were selected on each patient's individual immunoglobulin and used as ligands to trace cells expressing the idiotypic immunoglobulin on their surface. We established a flow cytometry and immunofluorescence protocol to track clonotypic B cells and validated it in two independent monoclonal B cell systems. Using this method, we found clonotypic B cells in only one out of 15 myeloma patients. In view of the assay's validated sensitivity level of 10−3, this surprising data suggests that the abundance of such cells has been vastly overestimated in the past and that they apparently represent a very rare population in myeloma. Our novel tracing approach may open perspectives to isolate and analyze clonotypic B cells and determine their role in myeloma pathobiology.

Place, publisher, year, edition, pages
Public Library of Science , 2012. Vol. 7, no 2, article id e31998
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Clinical Medicine
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URN: urn:nbn:se:lnu:diva-101596DOI: 10.1371/journal.pone.0031998Scopus ID: 2-s2.0-84857473351OAI: oai:DiVA.org:lnu-101596DiVA, id: diva2:1536900
Available from: 2021-03-12 Created: 2021-03-12 Last updated: 2021-06-14Bibliographically approved

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Rahlff, Janina

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CiteExportLink to record
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