Genomic, biochemical, and phylogenetic evaluation of bacteria isolated from deep-sea sediment harboring methane hydratesShow others and affiliations
2022 (English)In: Archives of Microbiology, ISSN 0302-8933, E-ISSN 1432-072X, Vol. 204, no 4, article id 205Article in journal (Refereed) Published
Abstract [en]
Over half of the organic carbon on Earth's surface is trapped in marine sediment as methane hydrates. Ocean warming causes hydrate dissociation and methane leakage to the water column, rendering the characterization of microbes from hydrate depositions a pressing matter. Through genomic, phylogenetic, and biochemical assays, we characterize the first microorganisms isolated from the Rio Grande Cone (Brazil), reservoir responsible for massive methane releases to the water column. From sediment harboring rich benthic communities, we obtained 43 strains of Brevibacillus sp., Paenibacillus sp. and groups of Bacillus sp. Methane-enriched samples yielded strains of the Pseudomonas fluorescens complex, exhibiting fluorescent siderophore production and broad multi-carbon catabolism. Genomic characterization of a novel Pseudomonas sp. strain indicated 32 genes not identified in the closest related type-species, including proteins involved with mercury resistance. Our results provide phylogenetic and genomic insights on the first bacterial isolates retrieved from a poorly explored region of the South Atlantic Ocean.
Place, publisher, year, edition, pages
Springer, 2022. Vol. 204, no 4, article id 205
Keywords [en]
Cold seeps, Deep-sea, Marine sediment, Microbial ecology, Microbiology
National Category
Microbiology Oceanography, Hydrology and Water Resources Environmental Sciences
Research subject
Ecology, Microbiology
Identifiers
URN: urn:nbn:se:lnu:diva-111050DOI: 10.1007/s00203-022-02814-zISI: 000766573600001PubMedID: 35266047Scopus ID: 2-s2.0-85126078640Local ID: 2022OAI: oai:DiVA.org:lnu-111050DiVA, id: diva2:1647855
2022-03-292022-03-292023-02-02Bibliographically approved