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Smart bio-nano interface derived from zein protein as receptors for biotinyl moiety
Linnaeus University, Faculty of Health and Life Sciences, Department of Chemistry and Biomedical Sciences. (Linnaeus Ctr Biomat Chem, BMC;BBCL)ORCID iD: 0000-0002-3921-4204
Vignans Fdn Sci Technol & Res, India.ORCID iD: 0000-0002-2192-4750
Indian Inst Technol Madras, India.
Linnaeus University, Faculty of Health and Life Sciences, Department of Chemistry and Biomedical Sciences. (Linnaeus Ctr Biomat Chem, BMC;BBCL)ORCID iD: 0000-0002-0407-6542
2023 (English)In: Talanta: The International Journal of Pure and Applied Analytical Chemistry, ISSN 0039-9140, E-ISSN 1873-3573, Vol. 256, article id 124298Article in journal (Refereed) Published
Abstract [en]

Proteinaceous, tunable nanostructures of zein (prolamine of corn) were developed as biotinyl-specific receptors using a molecular imprinting technique. Sacrificial templates, such as latex beads (LB3) and anodized alumina membrane (AAM), have been used to control nanostructural patterns in biotin-imprinted zein (BMZ). Briefly, a methanolic solution of the zein-biotin complex was drop cast upon a self-organized LB3 and AAM templates on Au/quartz surfaces. Subsequent dissolution of these sacrificial templates affords highly oriented, predetermined, and uniformly grown hyperporous (300 nm) and nanowires (150 nm) motifs of zein (BMZ-LB3 and BMZ-AAM), as shown by scanning electron microscopy (SEM). Selective extraction of biotin molecular template cast-off site -selective biotin imprints within these zein nanostructures complementary to biotinyl moieties. Alternatively, biotin-imprinted zein nanoparticles (BMZ-Np) and thin film (BMZ-MeOH) were prepared by coacervation and drop casting methods, respectively. Density functional theoretical (DFT) studies reveal strong hydrogen-bonded interaction of biotin with serine and glutamine residues of zein. Quartz crystal microbalance (QCM) studies show remarkable sensitivity of the hyperporous-BMZ-LB3 and nanowires of BMZ-AAM towards biotin derivative (biotin methyl ester) by five (24.75 +/- 1.34 Hz/mM) and four (18.19 +/- 0.75 Hz/mM) times, respectively, higher than the BMZ-MeOH. Enhanced permeability features of the zein nanostructures, when templated with LB3, enable the QCM detection of biotin-or its derivatives down to 12.9 ng mL-1 from dairy products (Kefir). The outcome of this study shall be a key aspect in interfacing biological materials with micro-/nano-sensors and electronic devices for detecting pertinent analytes using sustainably developed biopolymer-based nanostructures.

Place, publisher, year, edition, pages
Elsevier, 2023. Vol. 256, article id 124298
Keywords [en]
Zein, Protein nanostructures, Sacrificial template, Biotin-biosensor, Quartz crystal microbalance, Imprinted biopolymer
National Category
Biochemistry and Molecular Biology
Research subject
Chemistry, Biochemistry
Identifiers
URN: urn:nbn:se:lnu:diva-119764DOI: 10.1016/j.talanta.2023.124298ISI: 000926449800001PubMedID: 36701858Scopus ID: 2-s2.0-85146919249OAI: oai:DiVA.org:lnu-119764DiVA, id: diva2:1743876
Available from: 2023-03-16 Created: 2023-03-16 Last updated: 2023-05-31Bibliographically approved

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Suriyanarayanan, SubramanianNicholls, Ian A.

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