Start-ups are often born in some kind of ‘hosting’ environment such as business and university incubators or science technology parks. For decades, this has been considered an important measure of enhancing academic entrepreneurship (Grimaldi, Kenney, Siegel, & Wright, 2011) and the beginning of a start-up’s journey towards becoming a full-grown business. In this chapter, we aim to examine the challenges that start-ups meet when they begin to acquire the shape of a business venture and attempt to develop commercially viable business relationships with customers and suppliers.