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Kokta äggs hållbarhet i rumstemperatur.: Studie av lipidoxidation och mikrobiell förekomst.
Linnaeus University, Faculty of Health and Life Sciences, Department of Chemistry and Biomedical Sciences.
2023 (Swedish)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Sustainable development
SDG 12: Ensure sustainable consumption and production patterns
Alternative title
Shelf life of boiled eggs at room temperature. : Study of lipid oxidation and microbial occurrence. (English)
Abstract [en]

Eggs are a popular food widely consumed worldwide, due to their nutrient density and shelf life even at room temperature. This study aimed to investigate if eggs keep better in the refrigerator than at room temperature post-boiling. The shelf life of boiled eggs stored at room temperature and refrigerated was analyzed regarding lipid oxidation and microbiological growth. Lipid oxidation was determined by measuring thiobarbituric acid reaction substances (TBARS) at 532 nm, formed as the oxidation product malondialdehyde and thiobarbituric acid (TBA). Microbiological presence and growth were analyzed on egg yolk and egg white determining total counts of aerobic microorganisms on agar incubated for three days at room temperature. No difference in counts of aerobic microorganisms between boiled eggs stored at room temperature or in the refrigerator was detected for either lipid oxidation or microbiological growth. After 14 days, a difference in lipid oxidation was noted as compared to 7 days. The study found that boiled eggs have a long shelf life and can be stored at room temperature for 7 days.

Abstract [sv]

Ägg är ett omtyckt livsmedel som konsumeras flitigt i hela världen, och det är inte förvånande på grund av dess näringstäthet samt dess hållbarhet, även i rumstemperatur. Syftet med denna studie var att undersöka hur bra ägg håller i rumstemperatur efter kokning jämfört med i kylskåp. Hållbarheten på kokta ägg förvarade i rumstemperatur och kyl har analyserades med avseende på lipidoxidation och mikrobiologisk tillväxt. Lipidoxidation bestämdes genom att mäta thiobarbituric acid reactive substances (TBARS), vid 532nm, som bildas genom att oxidationsprodukten malondialdehyd reagerar med thiobarbituric acid (TBA). Mikrobiell förekomst och tillväxt i äggulan och äggvitan bestämdes på plate count agar efter inkubation i tre dagar i rumstemperatur. Ingen skillnad mellan kokta ägg förvarade i rumstemperatur eller kyl observerades för vare sig lipidoxidation eller mikrobiologisk tillväxt. Efter 14 dagar noterades en skillnad avseende lipidoxidationen i äggula jämfört med 7 dagar. Studien visade att kokta ägg har en lång hållbarhet och kan förvaras i rumstemperatur i upp till 7 dagar. 

Place, publisher, year, edition, pages
2023. , p. 17
Keywords [en]
egg, lipidoxidation, thiobarbituric acid, TBARS
Keywords [sv]
ägg, lipidoxidation, tiobarbitursyra, TBARS
National Category
Chemical Sciences Food Science
Identifiers
URN: urn:nbn:se:lnu:diva-123353OAI: oai:DiVA.org:lnu-123353DiVA, id: diva2:1783797
External cooperation
Thuressons hönseri AB
Subject / course
Chemistry
Educational program
Nutrition and Food Science Programme, 180 credits
Supervisors
Examiners
Available from: 2023-08-14 Created: 2023-07-24 Last updated: 2023-08-14Bibliographically approved

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CiteExportLink to record
Permanent link

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Citation style
  • apa
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Output format
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