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A novel and rapid method to quantify cytolytic replication of picornaviruses in cell culture
University of Kalmar, School of Pure and Applied Natural Sciences.
University of Kalmar, School of Pure and Applied Natural Sciences.
University of Kalmar, School of Pure and Applied Natural Sciences.
University of Kalmar, School of Pure and Applied Natural Sciences.ORCID iD: 0000-0003-3841-4826
2005 (English)In: Journal of virological methods, Vol. 130, p. 117-123Article in journal (Refereed) Published
Abstract [en]

Determining viral titers is a key issue in a wide variety of studies regarding different aspects of virology. The standard methods used for determining picornavirus titers are endpoint titration assay and plaque assay, both time consuming and laborious. The method described uses the tetrazolium salt MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide) that is reduced to formazane by cellular dehydrogenase, genes shown to be down-regulated during picornavirus infection. The amount formazane produced correlates with the viral titers obtained and can easily be measured using an ELISA plate reader. The colorimetric method has been evaluated using virus types from different genera of the Picornaviridae family. The MTT method reduces the time spent on determining the viral titers and still maintains a reliable accuracy.

Place, publisher, year, edition, pages
2005. Vol. 130, p. 117-123
National Category
Microbiology in the medical area
Research subject
Biomedical Sciences, Virology; Natural Science, Biomedical Sciences; Natural Science, Microbiology
Identifiers
URN: urn:nbn:se:lnu:diva-564DOI: 10.1016/j.jviromet.2005.06.016OAI: oai:DiVA.org:lnu-564DiVA, id: diva2:307294
Available from: 2010-04-01 Created: 2010-04-01 Last updated: 2018-01-12Bibliographically approved
In thesis
1. Tissue tropism and oncolytic potential of enteroviruses
Open this publication in new window or tab >>Tissue tropism and oncolytic potential of enteroviruses
2012 (English)Doctoral thesis, comprehensive summary (Other academic)
Alternative title[sv]
Vävnadstropism och onkolytisk potential hos enterovirus
Place, publisher, year, edition, pages
Växjö, Kalmar: Linnaeus University Press, 2012
Series
Linnaeus University Dissertations ; 77/2012
Keywords
picornavirus, enterovirus, echovirus, receptor, tropism, viral oncolysis, quantification, receptor interactions, colon cancer
National Category
Other Natural Sciences
Research subject
Natural Science, Biomedical Sciences
Identifiers
urn:nbn:se:lnu:diva-17721 (URN)978-91-86983-32-1 (ISBN)
Public defence
2012-03-09, N2007, Smålandsgatan 26B, Kalmar, 09:00 (Swedish)
Opponent
Supervisors
Available from: 2012-02-28 Created: 2012-02-22 Last updated: 2012-02-28Bibliographically approved

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Johannesson, PerIsraelsson, StinaEdman, KjellLindberg, A Michael

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