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Plant Cell Culture using a Novel Bioreactor: The Magnetically Stabilized Fluidized Bed
University of Kalmar, School of Pure and Applied Natural Sciences.ORCID iD: 0000-0001-8899-5046
1990 (English)In: Biotechnology progress (Print), ISSN 8756-7938, E-ISSN 1520-6033, Vol. 6, no 6, p. 452-457Article in journal (Refereed) Published
Abstract [en]

A novel bioreactor using magnetically stabilized fluidized bed (MSFB) technology has been developed that has certain advantages for cultivating cells continuously. In this system, the cells are protected from shear and are constrained to move through the fermenter in lock-step fashion by being immobilized in calcium alginate beads. The MSFB permits good mass transfer, minimizes particle collisions, and allows for the production of cells while maintaining a controlled cell residence time. Details of the experimental system are described. In addition, the experimental performance of an MSFB used to grow plant cells in batch mode is compared to the results obtained in shake flask culture. 

Place, publisher, year, edition, pages
1990. Vol. 6, no 6, p. 452-457
National Category
Biochemistry and Molecular Biology
Research subject
Natural Science, Biochemistry
Identifiers
URN: urn:nbn:se:lnu:diva-582DOI: 10.1021/bp00006a008OAI: oai:DiVA.org:lnu-582DiVA, id: diva2:307312
Available from: 2010-04-01 Created: 2010-04-01 Last updated: 2017-12-12Bibliographically approved

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Brodelius, Peter

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