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Immobilization of Plant Protoplasts: Viability Studies
UNIV LUND, CTR CHEM, S-22007 LUND 7, SWEDEN.ORCID iD: 0000-0001-8899-5046
1985 (English)In: Plant Cell Reports, ISSN 0721-7714, E-ISSN 1432-203X, Vol. 4, no 1, p. 23-27Article in journal (Refereed) Published
Abstract [en]

Protoplasts of Daucus carota Ca68 and Catharanthusroseus have been inmobilized by entrapment in gelformingpolysaccharides (kappa-carrageenan, agarose andalginate). Uniform spherical beads of carrageenan andagarose containing the protoplasts have been preparedby utilizing an inert hydrophobic phase (vegetableoil). The entrapped protoplasts are viable and stabilizedtowards osmotic shock by the polymeric backbone.Standard methods have been used to study the viabilityand integrity of the entrapped protoplasts. Uponincubation in a relatively simple medium the inmobilizedprotoplasts show a much higher viability after14 days as compared to free protoplasts under the sameconditions. The viability of D. carota protoplasts hasalso been monitored by an enzyme activity present inthe cells (digitoxigenin 58-hydroxylase) . 

Place, publisher, year, edition, pages
1985. Vol. 4, no 1, p. 23-27
National Category
Biochemistry and Molecular Biology
Research subject
Natural Science, Biochemistry
Identifiers
URN: urn:nbn:se:lnu:diva-591OAI: oai:DiVA.org:lnu-591DiVA, id: diva2:307321
Available from: 2010-04-01 Created: 2010-04-01 Last updated: 2017-12-12Bibliographically approved

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Brodelius, Peter

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