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Elicitor-induced Tyrosine Decarboxylase in Berberine Synthesizing Suspension Cultures of Thalictrum rugosum
Institute of Biotechnology, Swiss Federal Institute of Technology, Hönggerberg, CH-8093 Zfirich, Switzerland.ORCID iD: 0000-0001-8899-5046
1988 (English)In: European Journal of Biochemistry, ISSN 0014-2956, E-ISSN 1432-1033, Vol. 170, no 3, p. 661-666Article in journal (Refereed) Published
Abstract [en]

Tyrosine decarboxylase (EC 4.1.1.25) was induced in suspension cultures of Thalictrum rugosum by treatment with a yeast glucan elicitor. Maximum induction was observed at a carbohydrate concentration of 0.4 mg/g fresh weight of cells and maximum enzyme activity was reached 20 h after addition of elicitor. The enzyme was inducible in late exponential and early stationary growth phases. A good correlation between induced tyrosine decarboxylase activity and berberine biosynthesis has been established. It is suggested that tyrosine decarboxylase may be a key enzyme between primary and secondary metabolisms in the biosynthesis of norlaudanosoline-derived alkaloids. 

Place, publisher, year, edition, pages
1988. Vol. 170, no 3, p. 661-666
National Category
Biochemistry and Molecular Biology
Research subject
Natural Science, Biochemistry
Identifiers
URN: urn:nbn:se:lnu:diva-599DOI: 10.1111/j.1432-1033.1988.tb13748.xOAI: oai:DiVA.org:lnu-599DiVA, id: diva2:307329
Available from: 2010-04-01 Created: 2010-04-01 Last updated: 2017-12-12Bibliographically approved

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Brodelius, Peter

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