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Determination of Dissociation Constants for Binary Dehydrogenase-Coenzyme Complexes by (Bio)Affinity Chromatography on an Immobilized AMP-Analogue
UNIV LUND, CTR CHEM, DEPT PURE & APPL BIOCHEM, S-22007 LUND 7, SWEDEN .ORCID iD: 0000-0001-8899-5046
1976 (English)In: Analytical biochemistry, Vol. 72, no 1-2, p. 629-636Article in journal (Refereed) Published
Abstract [en]

Various alcohol and lactate dehydrogenases were adsorbed to an affinity column of immobilized N6-(6-aminohexyl)-AMP and subsequently eluted with gradients of coenzymes or coenzyme fragments. A linear relation was observed between the eluting concentration of nucleotide and the reported dissociation constants for the corresponding binary enzyme-nucleotide complexes. This relation has been utilized to determine unknown dissociation constants by affinity chromatography. The method presented can also be utilized for the estimation of dissociation constants betweendehydrogenases and coenzyme analogues. 

Place, publisher, year, edition, pages
1976. Vol. 72, no 1-2, p. 629-636
National Category
Biochemistry and Molecular Biology
Research subject
Natural Science, Biochemistry
Identifiers
URN: urn:nbn:se:lnu:diva-611DOI: 10.1016/0003-2697(76)90576-5OAI: oai:DiVA.org:lnu-611DiVA, id: diva2:307341
Available from: 2010-04-01 Created: 2010-04-01 Last updated: 2016-10-25Bibliographically approved

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Brodelius, Peter

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