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Isolation and DNA analysis of protoplasts from developing female gametophytes of Picea abies (Norway spruce)
SWEDISH UNIV AGR SCI, DEPT PATHOL, S-75007 UPPSALA, SWEDEN .
1986 (English)In: Canadian Journal of Botany, ISSN 0008-4026, E-ISSN 1480-3305, Vol. 64, no 1, p. 108-112Article in journal (Refereed) Published
Abstract [en]

Female gametophytes of Picea abies were collected during their growth period (June and July). Tissues were prepared for light microscope examination of their development and for protoplast isolation and further DNA measurements. From archegonia initiation to early embryo development, protoplasts could easily be obtained from such gametophytes. Stained and fixed nuclei, prepared from the protoplasts, were subjected to flow cytometry and their DNA content was recorded to be half that of seedling derived nuclei. 

Place, publisher, year, edition, pages
1986. Vol. 64, no 1, p. 108-112
National Category
Biological Sciences
Research subject
Natural Science, Cell and Organism Biology
Identifiers
URN: urn:nbn:se:lnu:diva-990OAI: oai:DiVA.org:lnu-990DiVA, id: diva2:307773
Available from: 2010-04-01 Created: 2010-04-01 Last updated: 2017-12-12Bibliographically approved

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Hakman, Inger

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