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The development of somatic embryos in tissue-cultures initiated from immature embryos of Picea abies (Norway spruce)
Institute of Physiological Botany, University of Uppsala, Box 540, S-751 21 Uppsala, Sweden.
1985 (English)In: Plant Science, ISSN 0168-9452, E-ISSN 1873-2259, Vol. 38, no 1, p. 53-59Article in journal (Refereed) Published
Abstract [en]

Embryos of Picea abies at various developmental stages were cultured on defined media supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) (10−5 M) and N6-benzyladenine (BA) (5×10−6 M). The immature embryos gave rise to a highly friable and embryogenic callus which could be maintained by subculture and contained polarized and organized structures (somatic embryos) consisting of long highly vacuolated cells at one end (suspensor) and a group of small meristematic cells at the other (embryonal end). These structures closely resembled the early stages of normal zygotic embryogeny. Upon further culture these structures formed a bipolar shoot-root axis with an independent and closed vascular system. In many instances either the shoot or the root meristems failed to differentiate. Embryogenic tissues obtained on agar media could be transferred to liquid media and maintained by subculture for at least 6 months. The development of somatic embryos was observed in the liquid cultures also. 

Place, publisher, year, edition, pages
1985. Vol. 38, no 1, p. 53-59
National Category
Biological Sciences
Research subject
Natural Science, Cell and Organism Biology
Identifiers
URN: urn:nbn:se:lnu:diva-993DOI: 10.1016/0168-9452(85)90079-2OAI: oai:DiVA.org:lnu-993DiVA, id: diva2:307776
Available from: 2010-04-01 Created: 2010-04-01 Last updated: 2017-12-12Bibliographically approved

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Hakman, Inger

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