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Receptor-mediated uptake of horseradish peroxidase in innervated and denervated skeletal muscle
Department of Pharmacology, University of Lund, S-223 62, Lund, Sweden.
1985 (English)In: Experimental Cell Research, ISSN 0014-4827, E-ISSN 1090-2422, Vol. 160, no 1, p. 95-105Article in journal (Refereed) Published
Abstract [en]

The in vitro uptake of [3H]inulin and horseradish peroxidase (HRP) has been studied in innervated and 6 days denervated extensor digitorum longus muscle of the mouse. Both markers were taken up at a higher rate in denervated muscle. The increase in uptake after denervation was, however, larger for HRP than for [3H]inulin. After 2 h incubation at 37 °C, pH 7.3, in the presence of equimolar concentrations of HRP and [3H]inulin (approx. 2.1 μM), the uptake of HRP was approx. 8 times as great as the uptake of [3H]inulin in the same innervated muscles. In denervated muscle the HRP uptake was approx. 19 times as great as the [3H]inulin uptake in the same muscles. Various possible explanations of these differences in uptake have been considered and tested experimentally. [3H]Inulin uptake in skeletal muscle has previously been shown to obey bulk kinetics. The present investigation shows the HRP uptake to obey saturation kinetics. The HRP uptake shows dependency on divalent cations and is reduced if incubation is carried out at pH 6.4. The uptake of HRP, when used at a low, non-saturating concentration (10 μg/ml approx. 0.25 μM), is inhibited 60% by yeast mannan (0.1 mg/ml), ribonuclease B (0.1 mg/ml, approx. 7.4 μM), mannose (30 mM), monodansylcadaverine (1 mM), chloroquine (100 μM), trifluoperazine (25 μM) or maleic acid (2 mM). It is concluded that HRP is taken up in innervated and denervated skeletal muscle by a process of receptor-mediated endocytosis and that this uptake is under neurotrophic control. 

Place, publisher, year, edition, pages
1985. Vol. 160, no 1, p. 95-105
National Category
Pharmacology and Toxicology
Research subject
Biomedical Sciences, Pharmacology
Identifiers
URN: urn:nbn:se:lnu:diva-1026DOI: 10.1016/0014-4827(85)90239-3OAI: oai:DiVA.org:lnu-1026DiVA, id: diva2:307809
Available from: 2010-04-01 Created: 2010-04-01 Last updated: 2018-01-12Bibliographically approved

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Tågerud, Sven

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