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Analysis of a Glucose-Containing Tetrasaccharide by High-Performance Liquid Affinity Chromatography
Perstorp Biolytica AB, Lund.
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1989 (English)In: Analytical Biochemistry, ISSN 0003-2697, E-ISSN 1096-0309, Vol. 182, no 1, p. 48-53Article in journal (Refereed) Published
Abstract [en]

In the present work we have explored conditions for using a pulsed amperometric detector for on-line analysis of oligosaccharides eluted from a high-performance liquid affinity chromatography column. A monoclonal antibody that specifically binds a glucose-containing oligosaccharide is coupled to a SelectiSphere-10-activated tresyl column. The system is eluted isocratically and easily detects 10 ng of the oligosaccharide with a linear response up to 250 ng. Analysis of both serum and urine samples from normal individuals and patients with acute pancreatitis gives a single retarded peak with a retention time identical to that of authentic (Glc)4. Retarded material pooled from several analyses of urine was positively identified as (Glc)4 by GC-MS analysis. As this method requires little cleanup and no chemical derivitization of the sample and is performed rapidly (less than 20 min) at sensitivities of at least 10μg/liter in biological fluids, it represents a substantial improvement over previous GC-MS, radioimmunoassay, and enzyme-linked immunoadsorbent assay methods used to determine (Glc)4. 

Place, publisher, year, edition, pages
1989. Vol. 182, no 1, p. 48-53
National Category
Natural Sciences
Research subject
Natural Science, Biotechnology
Identifiers
URN: urn:nbn:se:lnu:diva-1080DOI: 10.1016/0003-2697(89)90716-1OAI: oai:DiVA.org:lnu-1080DiVA, id: diva2:307869
Available from: 2010-04-01 Created: 2010-04-01 Last updated: 2017-12-12Bibliographically approved

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Ohlson, Sten

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