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High-Performance Liquid Affinity Chromatographic Separation of Mouse Monoclonal Antibodies with Protein A Silica
Perstorp Biolytica AB, S-223 70 Lund Sweden.
BioCarb AB, S-223 70 Lund Sweden.
1987 (English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 397, p. 207-212Article in journal (Refereed) Published
Abstract [en]

Protein A, a bacterial cell wall protein found in Staphylococcus aureus, has been widely used for the analysis of immunoglobulins. By attaching protein A to a microparticulate silica support, a rapid and efficient chromatographic sorbent has been created for the separation of monoclonal antibodies. Examples are given of rapid separations (within 10 min) of murine monoclonal antibodies, belonging to various IgG subclasses and including IgGl. The monoclonal antibodies were isolated with a high purity and with 60–90% recovery of activity. The high-performance liquid affinity chromatography technique based on protein A provides a useful method for monitoring monoclonal antibodies in crude samples, such as ascites and cell culture supernatants. 

Place, publisher, year, edition, pages
1987. Vol. 397, p. 207-212
National Category
Natural Sciences
Research subject
Natural Science, Biotechnology
Identifiers
URN: urn:nbn:se:lnu:diva-1088DOI: 10.1016/S0021-9673(01)85003-2OAI: oai:DiVA.org:lnu-1088DiVA, id: diva2:307877
Available from: 2010-04-01 Created: 2010-04-01 Last updated: 2017-12-12Bibliographically approved

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Ohlson, Sten

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