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Detecation of Circulating Immune Complexes by PEG Precipication Combinied with ELISA
Gambro AB, Box 10101, S-220 10 Lund, Sweden.
1985 (English)In: JIM - Journal of Immunological Methods, ISSN 0022-1759, E-ISSN 1872-7905, Vol. 77, no 1, p. 87-93Article in journal (Refereed) Published
Abstract [en]

An assay to detect circulating immune complexes is described and exemplified with serum from SLE and RA patients. The method is based on selective precipitation of immune complexes with PEG followed by a specific ELISA assay to detect the immunoglobulins present in the precipitate. The immunoglobulins of the precipitate were then monitored by their capacity to bind protein A and dDNA. With the PEG-protein A-ELISA procedure immune complexes were detected in 46% of the sera from SLE patients compared with 68% when using the PEG-DNA-ELISA. 

Place, publisher, year, edition, pages
1985. Vol. 77, no 1, p. 87-93
National Category
Medical and Health Sciences
Research subject
Natural Science, Biotechnology
Identifiers
URN: urn:nbn:se:lnu:diva-1089DOI: 10.1016/0022-1759(85)90186-3OAI: oai:DiVA.org:lnu-1089DiVA, id: diva2:307878
Available from: 2010-04-01 Created: 2010-04-01 Last updated: 2017-12-12Bibliographically approved

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Ohlson, Sten

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