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Development of an immunoassay for the detection of minute amounts of IgG-coated erythrocytes in whole blood: Its application for the assessment of Fc-mediated clearance of anti-D-coated erythrocytes in vivo
University Hospital, Uppsala.ORCID iD: 0000-0001-7888-1571
1989 (English)In: Vox Sanguinis, ISSN 0042-9007, E-ISSN 1423-0410, Vol. 57, no 3, p. 188-192Article in journal (Refereed) Published
Abstract [en]

A rapid and sensitive immunoassay for the detection of minute quantities of IgG-coated erythrocytes in whole blood was developed. Washed red blood cells were incubated in two steps with anti-human IgG antiserum followed by 125I-labelled protein A. The assay was able to detect amounts of sensitized erythrocytes as small as 0.5 ml of packed erythrocytes in a total blood volume of 5 liters and hematocrit 40%. A linear relation between increasing amounts of IgG-coated red cells in whole blood and the binding of 125I-labelled protein A was obtained. We applied the technique on the assessment of the removal of IgG anti-D-coated erythrocytes from the circulation of test individuals. T1/2 for the elimination of approximately 4 ml packed red cells sensitized with 62 μg of anti-D in 14 normal subjects was 20±5 min (mean±SEM). A splenectomized person did not clear the injected cells from the circulation during the test period of 70 min. If a standard curve was constructed the total blood volume in the test subjects could be calculated. This value correlated well (r = 0.99) with the blood volume calculated from the height and weight of the test individuals. 

Place, publisher, year, edition, pages
1989. Vol. 57, no 3, p. 188-192
National Category
Immunology in the medical area
Research subject
Biomedical Sciences, Immunology
Identifiers
URN: urn:nbn:se:lnu:diva-1109DOI: 10.1111/j.1423-0410.1989.tb00821.xOAI: oai:DiVA.org:lnu-1109DiVA, id: diva2:307898
Available from: 2010-04-01 Created: 2010-04-01 Last updated: 2018-01-12Bibliographically approved

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Nilsson Ekdahl, Kristina

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