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Endotoxin, epinephrine, glucagon, insulin and calcium ionophore A23187 modulation of pyruvate kinase activity in cultured rat hepatocytes
Department of Clinical Immunology and Transfusion Medicine, University Hospital, Uppsala.ORCID iD: 0000-0001-7888-1571
1990 (English)In: Acta chirurgica Scandinavica, Vol. 156, no 10, p. 677-681Article in journal (Refereed) Published
Abstract [en]

Altered glucose metabolism is one of the commonly observed sequelae of sepsis and septic shock. The present investigation was undertaken to determine the role of endotoxin (ET) upon hepatocyte glucoregulation, by measuring the activity of pyruvate kinase (PK), a key glycolytic enzyme. Hepatocytes were exposed to endotoxin concentrations known to occur in vivo during sepsis, i.e., from 1 X 10(-14) to 1 X 10(-8) g/ml. The alteration of the enzyme activities after addition of epinephrine, glucagon, insulin and calcium ionophore A23187 with and without ET preincubation were also examined. ET alone decreased the PK activity by 12% at all concentrations tested. The basal inhibition of the enzyme caused by epinephrine (-48%) was partially blocked by ET preincubation above 1 X 10(-10) g/ml. There were no ET-(glucagon, calcium ionophore, insulin) interaction. These in vitro results do not support pyruvate kinase as a site of hepatic enzyme regulation defect in endotoxaemia. 

Place, publisher, year, edition, pages
1990. Vol. 156, no 10, p. 677-681
National Category
Immunology in the medical area
Research subject
Biomedical Sciences, Immunology
Identifiers
URN: urn:nbn:se:lnu:diva-1115OAI: oai:DiVA.org:lnu-1115DiVA, id: diva2:307904
Available from: 2010-04-01 Created: 2010-04-01 Last updated: 2018-01-12Bibliographically approved

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Nilsson Ekdahl, Kristina

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