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Fractionation of rat liver plasma membrane regions by two-phase partitioning
Biochemistry, Chemical Centre, University of Lund.
1986 (English)In: Biochemical journal, Vol. 235, p. 685-691Article in journal (Refereed) Published
Abstract [en]

Rat liver plasma membranes, enriched in blood-sinusoidal or bile-canalicular regions by differential andsucrose-gradient centrifugation, were further purified by partitioning in an aqueous polymer two-phasesystem. This method separates membranes according to differences in surface properties rather than size anddensity. A several-fold increase in the ratio of leucine aminopeptidase (a bile-canalicular marker) and5'-nucleotidase to asialo-orosomucoid binding (a blood-sinusoidal marker) was obtained in one fraction,whereas another fraction gave a 2-3-fold increase in ratio of blood-sinusoidal to bile-canalicular markers.Furthermore, the markers for both regions of the plasma membrane, as well as markers for Golgi membranesand lysosomes, showed a heterogeneous behaviour on counter-current distribution. 

Place, publisher, year, edition, pages
1986. Vol. 235, p. 685-691
National Category
Medical and Health Sciences
Research subject
Natural Science, Biomedical Sciences; Natural Science, Optometry
Identifiers
URN: urn:nbn:se:lnu:diva-1396OAI: oai:DiVA.org:lnu-1396DiVA, id: diva2:308268
Available from: 2010-04-06 Created: 2010-04-06 Last updated: 2011-07-15Bibliographically approved

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Gierow, Peter

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