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Heterogeneity of smooth endoplasmic reticulum from rat liver studied by two-phase partitioning
Department of Biochemistry, University of Lund.
1989 (English)In: Biochemical Journal, ISSN 0264-6021, E-ISSN 1470-8728, Vol. 262, no 1, p. 55-61Article in journal (Refereed) Published
Abstract [en]

Smooth microsomal membranes, prepared from rat liver by sucrose-density-gradient centrifugation, were subfractionated by counter-current distribution in an aqueous two-phase system consisting of poly(ethylene glycol) and Dextran T500. A comparison of the distribution curves of marker enzymes, together with theoretically calculated curves, indicated the presence of at least five membrane subfractions, differing in the ratios of the marker enzymes. Glucose-6-phosphatase and arylesterase distributed in one manner, and NADPH-cytochrome c reductase and NADH-ferricyanide reductase in another. Evidence for further heterogeneities in the distribution of marker enzymes in smooth microsomes was obtained by analysing the membrane domain structure using a recently described method [Albertsson (1988) Q. Rev. Biophys. 21, 61-98]. Phenobarbital treatment did not influence the behaviour of the marker enzymes. 

Place, publisher, year, edition, pages
1989. Vol. 262, no 1, p. 55-61
National Category
Medical and Health Sciences
Research subject
Natural Science, Biomedical Sciences; Natural Science, Optometry
Identifiers
URN: urn:nbn:se:lnu:diva-1398OAI: oai:DiVA.org:lnu-1398DiVA, id: diva2:308270
Available from: 2010-04-06 Created: 2010-04-06 Last updated: 2017-12-12Bibliographically approved

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Gierow, Peter

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