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Isolation and growth of protoplasts from suspensions of Pinus contorta Dougl. ex Loud.
Uppsala Universitet.
1983 (English)In: Plant Cell Reports, Vol. 2, no 2, p. 92-94Article in journal (Refereed) Published
Abstract [en]

Cell suspensions were initiated from embryo derived calli of Pinus contorta. Some of these cell lines could be maintained in culture for at least one year without reduced growth. A high yield of protoplasts was obtained from the cell suspensions. The protoplasts started to divide after two days and cell clusters could be observed after about two weeks. The growth phase of the cell suspensions was very important for the division of protoplasts. Only protoplasts isolated from suspensions in an actively dividing phase were able to divide with a high frequency and to give rise to cell clusters. 

Place, publisher, year, edition, pages
1983. Vol. 2, no 2, p. 92-94
National Category
Biological Sciences
Research subject
Natural Science, Cell and Organism Biology
Identifiers
URN: urn:nbn:se:lnu:diva-1583DOI: 10.1007/BF00270174OAI: oai:DiVA.org:lnu-1583DiVA, id: diva2:308629
Available from: 2010-04-06 Created: 2010-04-06 Last updated: 2011-06-14Bibliographically approved

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Hakman, Inger

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