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Somatic embryogenesis and plant regeneration from suspension cultures of Picea glauca (White spruce).
Inst, of Physiological Botany, Univ. of Uppsala.
1988 (English)In: Physiologia Plantarum, ISSN 0031-9317, Vol. 72, no 3, p. 579-587.Article in journal (Refereed) Published
Abstract [en]

Plantlets were regenerated from long-term embryogenic cultures of Picea glauca (Moench) Voss. (White spruce). Embryogenic calli, initiated from immature zygotic embryos and maintained by monthly subculture for 16 months, were used to establish suspension cultures. Small somatic embryos were continuously produced in liquid culture medium containing auxin and cytokinin and the cultures showed a sustained regeneration capacity for >6 months. Somatic embryos propagated in the suspension cultures developed further into embryos bearing cotyledons, about 1 month after transfer to solidified medium containing abscisic acid. Electron microscopic examination revealed that storage nutrients, lipids, proteins and carbohydrates, accumulated in the somatic embryos during this treatment with abscisic acid (ABA). Upon subculture to medium lacking plant growth regulators such embryos could develop into small green plantlets. 

Place, publisher, year, edition, pages
1988. Vol. 72, no 3, p. 579-587.
National Category
Biological Sciences
Research subject
Natural Science, Cell and Organism Biology
Identifiers
URN: urn:nbn:se:lnu:diva-1585DOI: 10.1111/j.1399-3054.1988.tb09168.xOAI: oai:DiVA.org:lnu-1585DiVA, id: diva2:308631
Available from: 2010-04-06 Created: 2010-04-06 Last updated: 2011-07-15Bibliographically approved

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Hakman, Inger

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