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Exoproteolytic activity determined by flow injection analysis: potential importance for bacterial growth in coastal marine ponds
University of Kalmar, School of Pure and Applied Natural Sciences. (MPEA)ORCID iD: 0000-0001-7155-3604
1994 (English)In: Aquatic Living Resources, ISSN 0990-7440, E-ISSN 1765-2952, Vol. 7, no 1, p. 17-24Article in journal (Refereed) Published
Abstract [en]

The measurement of the fluorescent 4-methyl-7-coumarinylamine released from the hydrolysis of a non-fluorescent peptide model substrate by exoproteolytic enzymes has been adapted to flow injection analysis (FIA). FIA allows samples to be processed very quickly (less than 2 min. for triplicate determinations) with good sensitivity (< 0.1 muM) and reproducibility (relative standard deviation < 3 % at the 0.2 muM level). In a coastal marine pond, exoproteolytic activity was closely related to bacterioplankton biomass. The high activity measured in pond water (maximum velocity: V(M) almost-equal-to 1.46 to 2.54 muM.h-1) emphasizes the importance of dissolved protein hydrolysis for bacterial growth and for dissolved organic nitrogen cycling. The mean turnover time of dissolved peptides was 7.6 days, and amino acids liberated by exoproteolytic activity could potentially support, on average, 40 % of the bacterial nitrogen demand.

Place, publisher, year, edition, pages
1994. Vol. 7, no 1, p. 17-24
National Category
Biological Sciences
Research subject
Ecology, Aquatic Ecology
Identifiers
URN: urn:nbn:se:lnu:diva-1651DOI: 10.1051/alr:1994003ISI: A1994NC90100003OAI: oai:DiVA.org:lnu-1651DiVA, id: diva2:308697
Available from: 2010-04-06 Created: 2010-04-06 Last updated: 2020-01-29Bibliographically approved

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Legrand, Catherine

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