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Secreted frizzled related protein 1 (Sfrp1) and Wnt signaling in innervated and denervated skeletal muscle.
University of Kalmar, School of Pure and Applied Natural Sciences.
University of Kalmar, School of Pure and Applied Natural Sciences.
University of Kalmar, School of Pure and Applied Natural Sciences.
2008 (English)In: Journal of Molecular Histology, ISSN 1567-2379, E-ISSN 1567-2387, Vol. 39, no 3, p. 329-337Article in journal (Refereed) Published
Abstract [en]

Wnts are secreted proteins with functions in differentiation, development and cell proliferation. Wnt signaling has also been implicated in neuromuscular junction formation and may function in synaptic plasticity in the adult as well. Secreted frizzled-related proteins (Sfrps) such as Sfrp1 can function as inhibitors of Wnt signaling. In the present study a potential role of Wnt signaling in denervation was examined by comparing the expression levels of Sfrp1 and key proteins in the canonical Wnt pathway, Dishevelled, glycogen synthase kinase 3 beta and beta-catenin, in innervated and denervated rodent skeletal muscle. Sfrp1 mRNA and immunoreactivity were found to be up-regulated in mouse hemidiaphragm muscle following denervation. Immunoreactivity, detected by Western blots, and mRNA, detected by Northern blots, were both expressed in extrasynaptic as well as perisynaptic parts of the denervated muscle. Immunoreactivity on tissue sections was, however, found to be concentrated postsynaptically at neuromuscular junctions. Using beta-catenin levels as a readout for canonical Wnt signaling no evidence for decreased canonical Wnt signaling was obtained in denervated muscle. A role for Sfrp1 in denervated muscle, other than interfering with canonical Wnt signaling, is discussed.

Place, publisher, year, edition, pages
2008. Vol. 39, no 3, p. 329-337
National Category
Pharmacology and Toxicology
Research subject
Biomedical Sciences, Pharmacology
Identifiers
URN: urn:nbn:se:lnu:diva-1721DOI: 10.1007/s10735-008-9169-yOAI: oai:DiVA.org:lnu-1721DiVA, id: diva2:308769
Available from: 2010-04-06 Created: 2010-04-06 Last updated: 2018-01-12Bibliographically approved
In thesis
1. Gene and protein expression in denervated atrophic and hypertrophic skeletal muscle
Open this publication in new window or tab >>Gene and protein expression in denervated atrophic and hypertrophic skeletal muscle
2011 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Following denervation skeletal muscles change their functional and structural properties. Some changes resemble conditions in developing muscles and may be important for reinnervation. Due to inactivity following denervation most skeletal muscles loose muscle mass and become atrophic. The hemidiaphragm muscle, however, undergoes a phase of transient hypertrophy following denervation, gaining weight during the first 6-10 days followed by a decrease in weight. In this thesis the expression (mRNA, protein and protein phosphorylations) of potential factors involved in the regulation of muscle mass were examined in denervated hind-limb and hemidiaphragm muscles.

NIFK is a protein that associates with Ki67, a protein expressed predominantly in proliferating cells. The mRNA expression of NIFK was upregulated in denervated atrophic muscles but unaltered in denervated hypertrophic muscles, suggesting a potential role in the regulation of skeletal muscle mass (Paper I). p38 MAPK has previously been implicated in both anabolic and catabolic processes. Its substrate MK2 becomes phosphorylated at two sites, one of which is suggested to be important for nuclear export. MK2 phosphorylation at this site correlated with muscle weight in both atrophic and hypertrophic denervated muscles and may thus have a role in atrophy and hypertrophy (Paper III). Factors regulating protein synthesis are likely to play a role in atrophy and hypertrophy and many signaling pathways appear to converge on the formation of the translation initiation complex. The protein expression and phosphorylation status of several components in both Wnt and Akt signaling pathways indicate increased protein synthesis in denervated atrophic muscles as well as in denervated hypertrophic muscles (Papers II, IV and V). This suggests that increased protein degradation is more important than decreased protein synthesis for the loss of muscle mass in denervated atrophic muscles.

Place, publisher, year, edition, pages
Växjö, Kalmar: Linnaeus University Press, 2011
Series
Linnaeus University Dissertations ; 34/2011
Keywords
Skeletal muscle, Denervation, Hypertrophy, Atrophy, Gene expression, Protein expression, Protein phosphorylation, NIFK, Wnt, MK2, Akt signaling
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:lnu:diva-10411 (URN)978-91-86491-61-1 (ISBN)
Public defence
2011-03-11, N2007, Smålandsgatan 24, Kalmar, 09:00 (Swedish)
Supervisors
Available from: 2011-01-31 Created: 2011-01-28 Last updated: 2014-05-09Bibliographically approved

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Svensson, AnnaNorrby, MarleneTågerud, Sven

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