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Identification of Surface Active Oat Proteins Suitable for Oil-in-Water Emulsions
Linnaeus University, Faculty of Science and Engineering, School of Natural Sciences.
2010 (English)Independent thesis Advanced level (degree of Master (One Year)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Oat has a high nutritional value resulting in a progressive interest in oat products on the food market. However, oat proteins have technological shortcomings which limit the production of oat products. The aim of this project was to identify oat proteins with strong amphiphilic properties that can be used in food formulations. All proteins are more or less amphiphilic which can result in competitive adsorption to the oil-water interface in emulsions between protein species. In this thesis oat proteins were extracted and used to stabilize oil-in-water emulsions. The emulsion droplet size was determined by studying the emulsions in microscope. Emulsions were then centrifuged to get a cream layer (CL) and a water layer (WL). Sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional gel electrophoresis were used to determine molecular weights and isoelectric point (pI) for oat proteins from the CL, WL and oat extract. The gel electrophoresis results show that oat proteins from the CL have a dominant band at 14.5 kDa and pI values ranging from 4.5 to 6. The band at 14.5 kDa was not dominant at the WL and extract gels. The conclusion is that the most amphiphilic proteins in oats are small peptides with quite low pI values. Considering the molecular weight of these proteins they might be albumins or prolamins.

Place, publisher, year, edition, pages
2010. , 39 p.
Keyword [en]
oats, oat proteins, emulsions
National Category
Other Basic Medicine
Identifiers
URN: urn:nbn:se:lnu:diva-8678OAI: oai:DiVA.org:lnu-8678DiVA: diva2:353543
Presentation
(Swedish)
Uppsok
Physics, Chemistry, Mathematics
Supervisors
Examiners
Available from: 2010-09-28 Created: 2010-09-27 Last updated: 2010-09-28Bibliographically approved

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Citation style
  • apa
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  • ieee
  • modern-language-association-8th-edition
  • vancouver
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Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
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Output format
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  • text
  • asciidoc
  • rtf