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Two conformational forms of target bound iC3b which distinctively bind complement receptors 1 and 2 (CR1 and CR2) and two specific monoclonal antibodies.
Uppsala University.
Uppsala University.
Uppsala University.
Linnaeus University, Faculty of Science and Engineering, School of Natural Sciences.ORCID iD: 0000-0001-7888-1571
2010 (English)In: Upsala Journal of Medical Sciences, Supplement, ISSN 0300-9726Article in journal (Other academic) Published
Abstract [en]

Introduction. The complement system is an essential part of the immune system of vertebrates. The central event of thecomplement activation cascade is the sequential proteolytic activation of C3, which is associated with profound alterations inthe molecule’s structure and conformation and is responsible for triggering most of the biological effects of complement.Material and methods. Here, we have studied the conformation of C3 fragments deposited onto an IgG-coated surface fromhuman serum during complement activation, using a set of unique monoclonal antibodies (mAbs) that are all specific for theC3dg portion of bound iC3b.Results. We were able to identify two conformational forms of target-bound iC3b: the first recognized by mAb 7D18.1, and thesecond by mAb 7D323.1. The first species of iC3b bound recombinant complement receptor 1 (CR1), while the second boundCR2. Since CR1 and CR2 are expressed by different subsets of leukocytes, this difference in receptor-binding capacity impliesthat there is a biological difference between the two forms of surface-bound iC3b.Conclusion. We propose that mAbs 7D18.1 and 7D323.1 can act as surrogate markers for CR1 and CR2, respectively, and thatthey may be useful tools for studying the immune complexes that are generated in various autoimmune diseases.

Place, publisher, year, edition, pages
2010.
Keyword [en]
Complement C3, complement receptors, immunoglobulin G, monoclonal antibodies, neo-epitopes
National Category
Biochemistry and Molecular Biology
Research subject
Natural Science, Biomedical Sciences
Identifiers
URN: urn:nbn:se:lnu:diva-10054DOI: 10.3109/03009734OAI: oai:DiVA.org:lnu-10054DiVA: diva2:388200
Available from: 2011-01-17 Created: 2011-01-17 Last updated: 2016-11-11Bibliographically approved

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Nilsson Ekdahl, Kristina
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CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf