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A filtrometric study on red blood cells after storage in frozen form
Department of Internal Medicine, University Hospital, Lund, Sweden.
Department of Internal Medicine, University Hospital, Lund, Sweden.
1994 (English)In: Clinical Hemorheology, ISSN 0271-5198/94, Vol. 14, no 6, 831-840 p.Article in journal (Refereed) Published
Abstract [en]

Abstract

 Background: For transfusion purpose, red blood cells can be frozen with glycerol for a long time. After thawing the red cells are washed, the intracellular glycerol is exchanged with water, and then the cells can be transfused. Most of the red blood cells that have been frozen have a potential for normal longterm survival after transfusion, but some of them become altered during the preservation procedure and are removed from the recipient's circulation, one possible mechanism for this being trapping of red cells in the spleen due to reduced deformability.

The aim of this study was to investigate the sensitivity of the St George's filtrometer to detect possible deformability defects in frozen, thawed and deglycerolized red blood cells.

Results: The suspension of deglycerolized cells showed a significant reduction in filterability indicating the presence of cells with reduced deformability. IrFR was decreased (p<0.05), RCTT increased (p<0.05) and CP increased (p<0.001) .

We found a reduction of filterability in these cells roughly corresponding to the presence of 0.01-0.1% glutaraldehyde treated cells. When 10% of the erythrocytes in a blood sample were hemolyzed and thus in ghost form IrFR and RCTT were unaffected, while CP was significantly increased (p<0.05) .

Conclusions: A suitable sampling technique may, however, minimize the influence from ghosts in blood samples used for filtrometry after freezing. Thus, in evaluating different freezing techniques filtrometry with the St George's instrument seems to have a high capacity to detect and quantify preservation injuries leading to decreased deformability in the red blood cells.

Key words: Blood storage; Erythrocyte deformability; Erythrocyte membrane; Glycerol; Hemorheology; Transfusion.

 

Place, publisher, year, edition, pages
New York, USA: Pergamon Press , 1994. Vol. 14, no 6, 831-840 p.
Keyword [en]
Blood storage; Erythrocyte deformability; Erythrocyte membrane; Glycerol; Hemorheology; Transfusion
National Category
Medical and Health Sciences
Research subject
Natural Science, Biomedical Sciences; Health and Caring Sciences, Caring Science
Identifiers
URN: urn:nbn:se:lnu:diva-11707OAI: oai:DiVA.org:lnu-11707DiVA: diva2:417240
Available from: 2011-05-16 Created: 2011-05-16 Last updated: 2011-09-15Bibliographically approved
In thesis
1. Erythrocyte deformability studies by viscometry and filtrometry
Open this publication in new window or tab >>Erythrocyte deformability studies by viscometry and filtrometry
1994 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

DOCTORAL DISSERTATION 

Abstract

The present thesis concerns factors of major importance in filtrometric studies of human erythrocytes, e.g. influence of buffer media, importance of filtration pressure for filtration through micropores and possible effects of damaged and hemolyzed blood cells. The reproducibility of results from haemorheological studies has also been studied. Furthermore the rheological effects on erythrocytes from experimental, functional manipulation have been studied, e.g. by adding of digitalis glycosides and corticosteroids. Finally, the rheological properties of blood, that has been stored in frozen form, have been evaluated.

The first paperof this thesis shows that red cell morphology, flow behaviour and also the reproducibility of measurements are strongly dependent on the composition of the surrounding buffer medium.

Paper 2points out the importance of a carefully chosen filtration pressure to make the experimental settings to come as close to the in vivo capillary circulatory conditions as possible. Influence from variations in MCV is also demonstrated in this paper.

In paper 3the function of the sodium/potassium pump has, by adding of ouabain, been interfered with, thus creating changes of the intracellular ion and charge conditions. This, in turn, influences on blood viscosity. When incubating erythrocytes with calcium ions a more direct effect on erythrocyte deformability is seen.

In paper 4is described the complex effect of corticosteroids on flow properties of red blood cells. Thus it is shown that the over all effect of adding a corticosteroid to a suspension of blood cells seems to be a decreased viscosity, in spite of the fact that a reduced deformability of red blood cells can be seen as a parallel phenomenon.

Finally, paper 5indicates that the damage caused on red blood cells during preservation by glycerol and in frozen form may be of a haemorheological kind. Filtration through micropores seem: to be an adequate method for evaluation of damage caused to cells by freezing and parallels to the in vivo conditions in the spleen can be seen. The St George's Filtrometer, which was used in this study, seems to be able to find damaged cells in as low concentrations as 1/1000.

It is concluded that a buffer solution, with a small amount of albumin added should be used in studies on filterability of red blood cells. It is also concluded that the cells are sensible to the pressure conditions used in the filtration process. A negative pressure around 30 mm H20 seems to be suitable in this type of filtration studies. Haemorheological effects of digitalis glycosides and corticosteroids are elucidated and so is the effect of blood preservation through glycerol treatment and freezing on red blood cells.

Place, publisher, year, edition, pages
Lund, Sweden: Lunds Universitet, 1994. 110 p.
Series
ISRN LUMEDW/MEMA--1042--SE
Keyword
Erythrocyte deformability; viscometry; Filtrometry; Red cell membrane; Buffer; Filtration pressure; stress factors; Mean corpuscular volume; Sodium-potassium pump, Ouabain, Corticosteroids; Blood storage; Transfusion
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:lnu:diva-11785 (URN)
Public defence
1994-06-08, Föreläsningssal 3, Centralblocket, Universitetssjukhuset i Lund, Lund, 09:00 (Swedish)
Opponent
Supervisors
Note
Doktorsexamen i experimentell medicinAvailable from: 2011-06-07 Created: 2011-05-19 Last updated: 2011-06-07Bibliographically approved

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