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Effects of adenosine A2A and A2B receptor activation on signalling pathways and cytokine production in the human urothelium
Linnaeus University, Faculty of Science and Engineering, School of Natural Sciences.
Linnaeus University, Faculty of Science and Engineering, School of Natural Sciences.
(English)Manuscript (preprint) (Other academic)
Abstract [en]

Extracellular adenosine is formed in response to inflammation and the adenosine A2A and A2Breceptor subtypes have both been implicated in modulation of inflammation. In the presentstudy, we examined adenosine A2A and A2B receptor expression and signalling pathways inhuman uroepithelial cells. Modulation of adenosine A2A and A2B receptor activation on theuropathogenic Escherichia coli (UPEC)‐stimulated IL‐8 host response was also evaluated. Thehuman uroepithelial cell line UROtsa was grown in cell culture and stimulated with a mixture ofpro‐inflammatory cytokines (CM) or UPEC. Receptor expression was examined by RT‐PCR andIL‐8, intracellular cAMP and phosphoproteins were measured by ELISA, EIA and multipleximmunoassay (Luminex), respectively. The mRNA expression of the adenosine A2A, but not A2B,receptor was up‐regulated in response to CM and UPEC. The adenosine A2A receptor agonist, CGS21680 did not stimulate cAMP production but CREB phosphorylation was slightly increased. Bycontrast, the adenosine A2 receptor agonist CPCA induced a pronounced cAMP and CREBresponse. Furthermore, adenosine A2A, but not A2B, receptor activation decreased ERK1/2, JNK,p38 and STAT3 phosphorylation. UPEC‐infection stimulated the host IL‐8 production but CPCAor CGS 21680 had no impact on basal or UPEC‐evoked IL‐8 production. In conclusion, our dataidentified marked differences in signalling pathways by activation of the adenosine A2A and A2Breceptors. Activation of the adenosine A2A receptor inhibited STAT3 and MAPK‐signalling, whilethe cAMP‐CREB pathway was induced by adenosine A2B receptor activation. No anti‐ or proinflammatoryeffects were found for uroepithelial adenosine A2A or A2B receptors.

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Medical and Health Sciences
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Natural Science, Biomedical Sciences
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URN: urn:nbn:se:lnu:diva-11744OAI: oai:DiVA.org:lnu-11744DiVA, id: diva2:417837
Available from: 2011-12-16 Created: 2011-05-18 Last updated: 2011-12-16Bibliographically approved

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Säve, SusannePersson, Katarina

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CiteExportLink to record
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Citation style
  • apa
  • ieee
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