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Column technique: A way to assess erythrocyte deformability?
Department of Internal Medicine, University Hospital, Lund, Sweden.
1992 (English)In: 2:a Nordiska Hemoreologisymposiet, Uppsala, Sweden, 3-4 april 1992, 1992Conference paper, Published paper (Other academic)
Abstract [en]

Abstract

 Methods: The technique used was a modification of Beutlerns method using an alfa-cellulose + sigmacell 50 column (6.4 ml in a 10 ml syringe, diameter16 mm) with choline-Ringer buffer. The time for erythrocytes to pass through the column was registered. Whole blood viscosity was measured at 37 OC in a computer controlled rotational Couette-type viscometer at standardized hematocrit 45%.

Material: Venous blood from two different groups of subjects were used. 10 hyperthyroid patients (increased blood viscosity) were compared to 10 healthy subjects (normal blood viscosity).

Results: Whole blood viscosity in the hyperthyroid patient group was increased at all shear rates (p<0.001) compared to healthy subjects. The mean passage time through the column for erythrocytes from thyrotoxic patients was 254+ 6.9 sec. and was significantly higher (p<0.025) compared to the passage time for normal subjects (219+ 21.5 sec.) The column passage time was strongly correlated to whole blood viscosity at all shear rates investigated in both groups (p<0.01- 0.001).

Conclusion: There is a correlation between erythrocyte passage time through an alfa-cellulose + sigmacell 50 column and whole blood viscosity. Is this a way to assess erythrocyte deformability, aggregability or what else?

 

Place, publisher, year, edition, pages
1992.
National Category
Medical and Health Sciences
Research subject
Natural Science, Biomedical Sciences
Identifiers
URN: urn:nbn:se:lnu:diva-11857OAI: oai:DiVA.org:lnu-11857DiVA, id: diva2:418560
Available from: 2011-05-23 Created: 2011-05-23 Last updated: 2011-09-14Bibliographically approved

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