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p38 mitogen activated protein kinase regulates exocrine secretion in rabbit lacrimal gland.
Linnaeus University, Faculty of Science and Engineering, School of Natural Sciences.
Linnaeus University, Faculty of Science and Engineering, School of Natural Sciences.
(English)Manuscript (preprint) (Other academic)
Identifiers
URN: urn:nbn:se:lnu:diva-13660OAI: oai:DiVA.org:lnu-13660DiVA: diva2:432880
Available from: 2011-08-08 Created: 2011-08-08 Last updated: 2012-01-03Bibliographically approved
In thesis
1. Effects of adenosine and acetylcholine on the lacrimal gland
Open this publication in new window or tab >>Effects of adenosine and acetylcholine on the lacrimal gland
2011 (English)Doctoral thesis, comprehensive summary (Other academic)
Alternative title[sv]
Effekter av adenosin och acetylkolin på tårkörteln
Abstract [en]

A balanced tear film is essential for a healthy ocular surface. Insufficient tear production may result in dry eye, a common disorder in the elderly population. Dry eye causes significant discomfort in the patients and may lead to visual impairment and ocular infections. The lacrimal gland secretes water, proteins and electrolytes to the aqueous layer of the tear film. Lacrimal gland secretion is tightly regulated by e.g. neuronally released acetylcholine. The effect of acetylcholine on lacrimal gland secretion was recently found to be potentiated by adenosine. Adenosine is an important signaling molecule acting upon the adenosine receptors: A1, A2A, A2B and A3.

The aim of this thesis was to study effects of adenosine and acetylcholine on intracellular signaling pathways and lacrimal gland secretion. Cholinergic stimulation of secretion was shown to be regulated by the mitogen activated protein kinase p38, a protein previously not known to be involved in exocrine secretion. p38 was activated in response to cholinergic stimulation and inhibition of p38 significantly diminished cholinergic secretion.

When investigating adenosine effects, potentiation of cholinergic secretion was observed by activation of the A2B receptor in addition to the previously studied A1 receptor. An A2 receptor agonist increased cholinergic rabbit lacrimal gland protein secretion at several concentrations. The increase was inhibited by antagonism of the A2B receptor, but not the A2A receptor. When investigating the intracellular signaling pathways following adenosine and acetylcholine receptor activation, adenosine was shown to increase of cAMP levels. An additional increase in cAMP levels was observed after parallel adenosine and cholinergic receptor activation. Inhibition of Ca2+ release from the endoplasmic reticulum had inhibitory effects of cholinergic stimulation of secretion. In addition, the expression of adenosine receptors in a mouse model of autoimmune dry eye was investigated. The results showed a lymphocyte dependent upregulation of A2A receptors in diseased mice compared to controls.

In conclusion, the results in this thesis provide significant contributions in the search of dry eye therapeutics through studies of adenosine and acetylcholine receptor activation.

Place, publisher, year, edition, pages
Växjö, Kalmar: Linnaeus Univeristy Press, 2011. 76 p.
Series
Linnaeus University Dissertations, 59/2011
Keyword
Lacrimal gland, adenosine, adenosine receptors, signal transduction, exocrine secretion, tear film, dry eye, Sjögren’s syndrome, acetylcholine, mitogen activated protein kinase, p38
National Category
Cell and Molecular Biology Ophthalmology Biochemistry and Molecular Biology Cell Biology Biochemistry and Molecular Biology
Research subject
Natural Science, Biomedical Sciences
Identifiers
urn:nbn:se:lnu:diva-13636 (URN)978-91-86491-97-0 (ISBN)
Public defence
2011-09-09, Sal N2007, Smålandsgatan 26B, Kalmar, 09:30 (English)
Opponent
Supervisors
Available from: 2011-08-09 Created: 2011-08-01 Last updated: 2011-08-09Bibliographically approved

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