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DNA phosphorothioation is widespread and quantized in bacterial genomes
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2011 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 108, 2963-2968 p.Article in journal (Refereed) Published
Abstract [en]

 Phosphorothioate (PT) modification of DNA, with sulfur replacing a nonbridging phosphate oxygen, was recently discovered as a product of the dnd genes found in bacteria and archaea. Given our limited understanding of the biological function of PT modifications, including sequence context, genomic frequencies, and relationships to the diversity of dnd gene clusters, we under-took a quantitative study of PT modifications in prokaryotic genomes using a liquid chromatography-coupled tandem quadrupole mass spectrometry approach. The results revealed a diversity of unique PT sequence contexts and three discrete genomic frequencies in a wide range of bacteria. Metagenomic analyses of PT modifications revealed unique ecological distributions, and a phylogenetic comparison of dnd genes and PT sequence contexts strongly supports the horizontal transfer of dnd genes. These results are consistent with the involvement of PT modifications in a type of restriction-modification system with wide distribution in prokaryotes.

Place, publisher, year, edition, pages
2011. Vol. 108, 2963-2968 p.
National Category
Natural Sciences
Research subject
Natural Science, Aquatic Ecology
Identifiers
URN: urn:nbn:se:lnu:diva-14959DOI: 10.1073/pnas.1017261108OAI: oai:DiVA.org:lnu-14959DiVA: diva2:448031
Available from: 2011-12-16 Created: 2011-10-13 Last updated: 2015-09-04Bibliographically approved

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Pinhassi, Jarone
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School of Natural Sciences
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