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A membrane protein based biosensor: Use of a phosphate - H+ symporter membrane protein (Pho84) in the sensing of phosphate ions.
Central Food Technology Research Institute.
Linnaeus University, Faculty of Science and Engineering, School of Natural Sciences.ORCID iD: 0000-0002-0381-251X
Lund university.
Central Food Technology Research Institute.
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2011 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 27, no 1, 58-63 p.Article in journal (Refereed) Published
Abstract [en]

A label free biosensor for direct detection of inorganic phosphate based on potential-step capacitance measurements has been developed. The high-affinity Pho84 plasma membrane phosphate/proton symporter of Saccharomyces cerevisiae was used as a sensing element. Heterologously expressed and purified Pho84 protein was immobilized on a self-assembled monolayer (SAM) on a capacitance electrode. Changes in capacitance were recorded upon exposure to phosphate compared to the control substance, phosphate analogue methylphosphonate. Hence, even without the explicit use of lipid membranes, the Pho84 membrane protein could retain its capacity of selective substrate binding, with a phosphate detection limit in the range of the apparent in vivo K(m). A linear increase in capacitance was monitored in the phosphate concentration range of 5-25 mu M. The analytical response of the capacitive biosensor is in agreement with that the transporter undergoes significant conformational changes upon exposure to inorganic phosphate, while exposure to the analogue only causes minor responses.

Place, publisher, year, edition, pages
Elsevier, 2011. Vol. 27, no 1, 58-63 p.
Keyword [en]
capacitive biosensor, membrane protein, Pho84, phosphate, symporter
National Category
Natural Sciences
Research subject
Natural Science, Biochemistry
Identifiers
URN: urn:nbn:se:lnu:diva-16721DOI: 10.1016/j.bios.2011.06.024OAI: oai:DiVA.org:lnu-16721DiVA: diva2:476118
Projects
Biosensors based on the action of membrane proteins
Funder
Swedish Research Council, 348-2005-6286
Available from: 2012-01-11 Created: 2012-01-11 Last updated: 2017-02-10Bibliographically approved

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Samyn, Dieter R.Persson, Bengt L.
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CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf