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The intrinsic GTPase activity of the Gtr1 protein from Saccharomyces cerevisiae
Linnaeus University, Faculty of Science and Engineering, School of Natural Sciences. (Bengt Persson)
University of Gothenburg.
Lund University.
Linnaeus University, Faculty of Science and Engineering, School of Natural Sciences. (Bengt Persson)ORCID iD: 0000-0002-0381-251X
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2012 (English)In: BMC Biochemistry, ISSN 1471-2091, E-ISSN 1471-2091, Vol. 13, 11Article in journal (Refereed) Published
Abstract [en]

Background

The Gtr1 protein of Saccharomyces cerevisiae is a member of the RagA subfamily of the Ras-like small GTPase superfamily. Gtr1 has been implicated in various cellular processes. Particularly, the Switch regions in the GTPase domain of Gtr1 are essential for TORC1 activation and amino acid signaling [R. Gong, L. Li, Y. Liu, P. Wang, H. Yang, L. Wang, J. Cheng, K.L. Guan, Y. Xu, Genes Dev. 25 (2011) 1668–1673]. Therefore, knowledge about the biochemical activity of Gtr1 is required to understand its mode of action and regulation.

Results

By employing tryptophan fluorescence analysis and radioactive GTPase assays, we demonstrate that Gtr1 can adopt two distinct GDP- and GTP-bound conformations, and that it hydrolyses GTP much slower than Ras proteins. Using cysteine mutagenesis of Arginine-37 and Valine-67, residues at the Switch I and II regions, respectively, we show altered GTPase activity and associated conformational changes as compared to the wild type protein and the cysteine-less mutant.

Conclusions

The extremely low intrinsic GTPase activity of Gtr1 implies requirement for interaction with activating proteins to support its physiological function. These findings as well as the altered properties obtained by mutagenesis in the Switch regions provide insights into the function of Gtr1 and its homologues in yeast and mammals.

Place, publisher, year, edition, pages
BioMed Central, 2012. Vol. 13, 11
Keyword [en]
Gtr1, GTPase, Intrinsic tryptophan fluorescence, Rag GTPase, Cysteine mutagenesis, Switch region
National Category
Biochemistry and Molecular Biology
Research subject
Chemistry, Biochemistry
Identifiers
URN: urn:nbn:se:lnu:diva-20450DOI: 10.1186/1471-2091-13-11OAI: oai:DiVA.org:lnu-20450DiVA: diva2:536992
Projects
Phosphate sensing and signaling in yeast
Funder
Swedish Research Council, 621-2003-3558 and 621-2007-6144
Available from: 2012-06-25 Created: 2012-06-25 Last updated: 2017-02-10Bibliographically approved

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CiteExportLink to record
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