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Effect of pH and sulfate concentration on hydrogen production using anaerobic mixed microflora
Department of Environmental Engineering, Yonsei University, Wonju, Gangwon-do, 220-710, South Korea.
Department of Environmental Engineering, Yonsei University, Wonju, Gangwon-do, 220-710, South Korea.
Department of Environmental Engineering, Yonsei University, Wonju, Gangwon-do, 220-710, South Korea / Department of Environmental Biotechnology, Mubarak City for Scientific Research, Alexandria, Egypt.
Department of Environmental Engineering, Yonsei University, Wonju, Gangwon-do, 220-710, South Korea. (ESEG)ORCID iD: 0000-0002-3565-9943
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2009 (English)In: International journal of hydrogen energy, ISSN 0360-3199, E-ISSN 1879-3487, Vol. 34, no 24, 9702-9710 p.Article in journal (Refereed) Published
Abstract [en]

The effects of varying sulfate concentrations with pH on continuous fermentative hydrogen production were studied using anaerobic mixed cultures growing on a glucose substrate in a chemostat reactor. The maximum hydrogen production rate was 2.8 L/day at pH 5.5 and sulfate concentration of 3000 mg/L. Hydrogen production and residual sulfate level decreased with increasing the pH from 5.5 to 6.2. The volatile fatty acids (VFAs) and ethanol fractions in the effluent were in the order of butyric acid (HBu) > acetic acid (HAc) > ethanol > propionic acid (HPr). Fluorescence In Situ Hybridization (FISH) analysis revealed the presence of hydrogen producing bacteria (HPB) under all pH ranges while sulfate reducing bacteria (SRB) were present at pH 5.8 and 6.2. The inhibition in hydrogen production by SRB at pH 6.2 diminished entirely by lowering to pH 5.5, at which activity of SRB is substantially suppressed.

Place, publisher, year, edition, pages
2009. Vol. 34, no 24, 9702-9710 p.
National Category
Environmental Sciences
Research subject
Natural Science, Environmental Science
Identifiers
URN: urn:nbn:se:lnu:diva-21077DOI: 10.1016/j.ijhydene.2009.10.022OAI: oai:DiVA.org:lnu-21077DiVA: diva2:543661
Available from: 2012-08-09 Created: 2012-08-09 Last updated: 2017-12-07Bibliographically approved

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Bhatnagar, Amit

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