lnu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Human neural progenitor cells promote photoreceptor survival in retinal explants
Department of Ophthalmology, Clinical Sciences, Lund, University of Lund, Sweden..
Linnaeus University, Faculty of Science and Engineering, School of Natural Sciences. (Retinal research)
Department of Ophthalmology, Clinical Sciences, Lund, University .
Department of Ophthalmology, Clinical Sciences, Lund, University .
Show others and affiliations
2010 (English)In: Experimental Eye Research, ISSN 0014-4835, E-ISSN 1096-0007, Vol. 90, no 2, 292-299 p.Article in journal (Refereed) Published
Abstract [en]

Different types of progenitor and stem cells have been shown to provide neuroprotection in animal models of photoreceptor degeneration. The present study was conducted to investigate whether human neural progenitor cells (HNPCs) have neuroprotective properties on retinal explants models with calpain- and caspase-3-dependent photoreceptor cell death. In the first experiments, HNPCs in a feeder layer were co-cultured for 6 days either with postnatal rd1 mouse or normal rat retinas. Retinal histological sections were used to determine outer nuclear layer (ONL) thickness, and to detect the number of photoreceptors with labeling for calpain activity, cleaved caspase-3 and TUNEL. The ONL thickness of co-cultured rat and rd1 retinas was found to be almost 10% and 40% thicker, respectively, compared to controls. Cell counts of calpain activity, cleaved caspase-3 and TUNEL labeled photoreceptors in both models revealed a 30-50% decrease when co-cultured with HNPCs. The results represent significant increases of photoreceptor survival in the co-cultured retinas. In the second experiments, for an identification of putative survival factors, or a combination of them, a growth factor profile was performed on conditioned medium. The relative levels of various growth factors were analyzed by densitometric measurements of growth factor array membranes. Following growth factors were identified as most potential survival factors; granulocyte colony stimulating factor (G-CSF), granulocyte-macrophage colony stimulating factor (GMCSF), insulin-like growth factor II (IGF-II), neurotrophic factor 3 (NT-3), placental growth factor (PIGF), transforming growth factors (TGF-beta1 and TGF-beta2) and vascular endothelial growth factor (VEGF-D). HNPCs protect both against calpain- and caspase-3-dependent photoreceptor cell death in the rd1 mouse and against caspase-3-dependent photoreceptor cell death in normal rat retinas in vitro. The protective effect is possibly achieved by a variety of growth factors secreted from the HNPCs.

Place, publisher, year, edition, pages
2010. Vol. 90, no 2, 292-299 p.
Keyword [en]
retina, photoreceptor, apoptosis, neuroprotection, progenitor cells
National Category
Natural Sciences
Research subject
Natural Science, Biomedical Sciences
Identifiers
URN: urn:nbn:se:lnu:diva-23045DOI: 10.1016/j.exer.2009.11.005OAI: oai:DiVA.org:lnu-23045DiVA: diva2:579059
Funder
EU, European Research Council
Available from: 2012-12-19 Created: 2012-12-19 Last updated: 2014-02-03Bibliographically approved
In thesis
1. Neural progenitor cell-derived neurotrophic support for the degenerating retina: an in vitro study
Open this publication in new window or tab >>Neural progenitor cell-derived neurotrophic support for the degenerating retina: an in vitro study
2013 (English)Doctoral thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Växjö: Linnaeus University press, 2013. 77 p.
Series
Linnaeus University Dissertations, 158
Keyword
retinal degeneration, apoptosis, retina, ER-stress, autophagy
National Category
Natural Sciences
Research subject
Natural Science, Biomedical Sciences
Identifiers
urn:nbn:se:lnu:diva-31164 (URN)978-91-87427-67-1 (ISBN)
Public defence
2013-12-20, N2007, Västergård, Kalmar, 09:00 (Swedish)
Opponent
Supervisors
Available from: 2014-02-03 Created: 2013-12-10 Last updated: 2014-02-03Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Mohlin, CamillaJohansson, Kjell
By organisation
School of Natural Sciences
In the same journal
Experimental Eye Research
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 92 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf