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Death of photoreceptors in organotypic retinal explant cultures: implication of rhodopsin accumulation and endoplasmic reticulum stress.
Linnaeus University, Faculty of Science and Engineering, School of Natural Sciences. (Retinal research)
Linnaeus University, Faculty of Science and Engineering, School of Natural Sciences.
2011 (English)In: Journal of Neuroscience Methods, ISSN 0165-0270, E-ISSN 1872-678X, ISSN 0165-0270, Vol. 197, no 1, 56-64 p.Article in journal (Refereed) Published
Abstract [en]

Here we suggest that endoplasmic reticulum (ER)-stress may be induced following aberrant rhodopsin accumulation in photoreceptors in explanted rat retinas. Rhodopsin accumulation was accompanied by increased phosphorylation of pancreatic ER-kinase and eukaryotic initiator factor 2α as well as increased levels of C/EBP homologous protein, glucose-regulated protein 78 and eventually increased cleaved caspase-12 and cleaved caspase-3. Glucose-regulated protein 78, pancreatic ER-kinase, caspase-12 and cleaved caspase-3 were present in photoreceptors, indicating that ER-stress and apoptosis are induced in this cell population. These results suggest that ER-stress and subsequent apoptosis is induced in healthy photoreceptors, presumably by aberrant accumulation of rhodopsin and the phosphorylation of eukaryotic initiator factor 2α. The explant culture system may allow investigations of neuroprotective strategies.

Place, publisher, year, edition, pages
2011. Vol. 197, no 1, 56-64 p.
Keyword [en]
Retina, Photoreceptor, Apoptosis, ER stress, Rhodopsin
National Category
Natural Sciences
Research subject
Natural Science, Biomedical Sciences
Identifiers
URN: urn:nbn:se:lnu:diva-23046DOI: 10.1016/j.jneumeth.2011.01.030OAI: oai:DiVA.org:lnu-23046DiVA: diva2:579080
Available from: 2012-12-19 Created: 2012-12-19 Last updated: 2014-02-03Bibliographically approved
In thesis
1. Neural progenitor cell-derived neurotrophic support for the degenerating retina: an in vitro study
Open this publication in new window or tab >>Neural progenitor cell-derived neurotrophic support for the degenerating retina: an in vitro study
2013 (English)Doctoral thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Växjö: Linnaeus University press, 2013. 77 p.
Series
Linnaeus University Dissertations, 158
Keyword
retinal degeneration, apoptosis, retina, ER-stress, autophagy
National Category
Natural Sciences
Research subject
Natural Science, Biomedical Sciences
Identifiers
urn:nbn:se:lnu:diva-31164 (URN)978-91-87427-67-1 (ISBN)
Public defence
2013-12-20, N2007, Västergård, Kalmar, 09:00 (Swedish)
Opponent
Supervisors
Available from: 2014-02-03 Created: 2013-12-10 Last updated: 2014-02-03Bibliographically approved

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Mohlin, CamillaJohansson, Kjell
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CiteExportLink to record
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Citation style
  • apa
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