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Determination of Sulfite in Beer Based on Fluorescent Derivatives and Liquid Chromatographic Separation
Linnaeus University, Faculty of Science and Engineering, School of Natural Sciences.
University of Copenhagen, Denmark.
University of Copenhagen, Denmark.
University of Copenhagen, Denmark.
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2012 (English)In: Journal of the American Society of Brewing Chemists, ISSN 0361-0470, E-ISSN 1943-7854, Vol. 70, no 4, 296-302 p.Article in journal (Refereed) Published
Abstract [en]

A method was developed for quantification of sulfite in beer based on derivatization with the maleimide-derived probe ThioGlo I followed by separation of fluorescent adducts by reversed-phase high-performance liquid chromatography and fluorescence detection. Sulfite gave two ThioGlo 1 derivatives and it was shown by mass spectrometry that both had identical mass spectra. Matrix effects were observed when constructing sulfite standard curves in different beers and, therefore, use of a matrix-matched calibration curve is proposed. ThioGlo I was found to generate fluorescent adducts with both bound and free sulfite, providing a quantification of the total sulfite content in beer. The limit of quantification of sulfite was 0.6 mg/L and the method can be used for quantification of sulfite in highly colored beers.

Place, publisher, year, edition, pages
2012. Vol. 70, no 4, 296-302 p.
Keyword [en]
Analysis, Beer, Matrix match, Sulfite, ThioGlo 1
National Category
Analytical Chemistry
Research subject
Chemistry, Organic Chemistry
Identifiers
URN: urn:nbn:se:lnu:diva-24502DOI: 10.1094/ASBCJ-2012-1012-01ISI: 000312628600011OAI: oai:DiVA.org:lnu-24502DiVA: diva2:607289
Available from: 2013-02-22 Created: 2013-02-22 Last updated: 2017-03-09Bibliographically approved

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CiteExportLink to record
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Citation style
  • apa
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