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Characterization of the biochemical and biophysical properties of the Saccharomyces cerevisiae phosphate transporter Pho89
Linnaeus University, Faculty of Health and Life Sciences, Department of Chemistry and Biomedical Sciences.
Lund university.
Lund university.
Linnaeus University, Faculty of Health and Life Sciences, Department of Chemistry and Biomedical Sciences.
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2013 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 436, no 3, 551-556 p.Article in journal (Refereed) Published
Abstract [en]

In Saccharomyces cerevisiae, Pho89 mediates a cation-dependent transport of Pi across the plasma membrane. This integral membrane protein belongs to the Inorganic Phosphate Transporter (PiT) family, a group that includes the mammalian Na+/Pi cotransporters Pit1 and Pit2. Here we report that the Pichia pastoris expressed recombinant Pho89 was purified in the presence of Foscholine-12 and functionally reconstituted into proteoliposomes with a similar substrate specificity as observed in an intact cell system. The alpha-helical content of the Pho89 protein was estimated to 44%. EPR analysis showed that purified Pho89 protein undergoes conformational change upon addition of substrate. 

Place, publisher, year, edition, pages
Elsevier , 2013. Vol. 436, no 3, 551-556 p.
Keyword [en]
Pho89, Pichia pastoris, Oligomer, Reconstitution, Phosphate transport, Circular dichroism
National Category
Chemical Sciences Biochemistry and Molecular Biology
Research subject
Chemistry, Biochemistry
Identifiers
URN: urn:nbn:se:lnu:diva-27720DOI: 10.1016/j.bbrc.2013.06.011ISI: 000321995900034OAI: oai:DiVA.org:lnu-27720DiVA: diva2:638664
Projects
Characterization of sensors and signal transduction in regulation of phosphate uptake systems
Funder
Swedish Research Council, 621-2007-6144Swedish Research Council, 522-2008-3724,7480
Available from: 2013-08-01 Created: 2013-08-01 Last updated: 2017-02-16Bibliographically approved

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Sengottaiyan, PalaniveluRuiz-Pavon, LorenaPersson, Bengt L.
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CiteExportLink to record
Permanent link

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Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
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